Janelia Fluor® 646, Maleimide  (Synonyms: JF646, Maleimide)

Janelia Fluor® 646, Maleimide (JF646, Maleimide) is a red fluorescent dye that contains a maleimide group. JF646, Maleimide can be used in cellular imaging^[1][2]. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

λmax (nm)=646; λem (nm)=664^[1].
JF646 can serve as a ligand for self-labeling tag (such as HaloTag)^[1].
JF646, Maleimide is modified by JF646. JF646, a red fluorescent dye, is photostable, membrane-permeable, has a high extinction coefficient^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

618.75

C₃₅H₃₄N₄O₅Si

Solid

Light green to green

646

605

O=C(C1=CC=C(C(C(C2=CC=C(C=C2[Si]3(C)C)N4CCC4)=C(C3=C/5)C=CC5=[N+]6CCC/6)=C1)C([O-])=O)NCCN7C(C=CC7=O)=O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Grimm JB, et al. A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat Methods. 2015;12(3):244-250. doi:10.1038/nmeth.3256  [Content Brief]

  [2]. Basu S, et al. FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells. Nat Commun. 2018;9(1):2520. Published 2018 Jun 28.  [Content Brief]