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Janelia Fluor® 646 TFA  (Synonyms: JF646 TFA)

Cat. No.: HY-131028 Purity: ≥95.0%
COA Handling Instructions

Janelia Fluor® 646 TFA (JF646 TFA), a red fluorogenic fluorescent dye, can be used in the synthesis of Janelia Fluor 646 HaloTag and SNAP-Tag ligands. JF646 TFA is used in live cell imaging experiments. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

For research use only. We do not sell to patients.

Janelia Fluor® 646 TFA Chemical Structure

Janelia Fluor® 646 TFA Chemical Structure

Size Price Stock Quantity
1 mg USD 205 In-stock
5 mg USD 452 In-stock
10 mg USD 768 In-stock
50 mg   Get quote  
100 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Janelia Fluor® 646 TFA:

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  • Biological Activity

  • Purity & Documentation

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Description

Janelia Fluor® 646 TFA (JF646 TFA), a red fluorogenic fluorescent dye, can be used in the synthesis of Janelia Fluor 646 HaloTag and SNAP-Tag ligands. JF646 TFA is used in live cell imaging experiments[1][2]. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

In Vitro

JF646 TFA: λmax (nm)=646, λem (nm) =664[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

610.65

Formula

C31H29F3N2O6Si

Appearance

Solid

Color

Blue to dark blue

Emission (Em)

646

Excitation (Ex)

605

SMILES

OC(C1=CC=C(C(C(C2=CC=C(C=C2[Si]3(C)C)N4CCC4)=C(C3=C/5)C=CC5=[N+]6CCC/6)=C1)C([O-])=O)=O.FC(C(O)=O)(F)F

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (163.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6376 mL 8.1880 mL 16.3760 mL
5 mM 0.3275 mL 1.6376 mL 3.2752 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6376 mL 8.1880 mL 16.3760 mL 40.9400 mL
5 mM 0.3275 mL 1.6376 mL 3.2752 mL 8.1880 mL
10 mM 0.1638 mL 0.8188 mL 1.6376 mL 4.0940 mL
15 mM 0.1092 mL 0.5459 mL 1.0917 mL 2.7293 mL
20 mM 0.0819 mL 0.4094 mL 0.8188 mL 2.0470 mL
25 mM 0.0655 mL 0.3275 mL 0.6550 mL 1.6376 mL
30 mM 0.0546 mL 0.2729 mL 0.5459 mL 1.3647 mL
40 mM 0.0409 mL 0.2047 mL 0.4094 mL 1.0235 mL
50 mM 0.0328 mL 0.1638 mL 0.3275 mL 0.8188 mL
60 mM 0.0273 mL 0.1365 mL 0.2729 mL 0.6823 mL
80 mM 0.0205 mL 0.1023 mL 0.2047 mL 0.5117 mL
100 mM 0.0164 mL 0.0819 mL 0.1638 mL 0.4094 mL
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Janelia Fluor® 646 TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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