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  3. Cy 5 hydrazide

Cy 5 hydrazide  (Synonyms: Cyanine5 hydrazide)

Cat. No.: HY-137100
Handling Instructions

Cy 5 hydrazide (Cyanine5 hydrazide) is a fluorescent dye for aldehydes and ketones. Cy 5 hydrazide can be used for labeling proteins.

For research use only. We do not sell to patients.

Cy 5 hydrazide Chemical Structure

Cy 5 hydrazide Chemical Structure

CAS No. : 1427705-31-4

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Description

Cy 5 hydrazide (Cyanine5 hydrazide) is a fluorescent dye for aldehydes and ketones. Cy 5 hydrazide can be used for labeling proteins[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Cell viability assay[1]:
1. Add Cy 5 hydrazide to each sample at 25℃ for 1 h to label sample.
2. Remove salts and unbound fluorescent dye by the trichloroacetic acid (TCA esodium deoxycholate (DOC) or acetone method.
3. Measure fluorescence intensity (Ex=640 nm, Em=660 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

569.61

Formula

C32H42Cl2N4O

CAS No.
Emission (Em)

670

Excitation (Ex)

645

SMILES

O=C(NN)CCCCC[N+](C1=CC=CC=C1C(C)2C)=C2/C=C/C=C/C=C(C3(C)C)\N(C4=CC=CC=C34)C.[Cl-].Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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Cy 5 hydrazide Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Cy 5 hydrazide
Cat. No.:
HY-137100
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