1. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (174):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-15534
    JC-1 3520-43-2
    JC-1, a fluorescent lipophilic carbocyanine dye, that can be used to measure mitochondrial membrane potential.
    JC-1
  • HY-12591A
    D-Luciferin 2591-17-5 98.11%
    D-luciferin is the natural substrate of luciferases that catalyze the production of light in bioluminescent insects.
    D-Luciferin
  • HY-15558
    Hoechst 33258 23491-44-3 99.13%
    Hoechst 33258 is a fluorescent dyes, which can be used as a cell dye for DNA.
    Hoechst 33258
  • HY-D0913
    1-Methyl-7-nitroisatoic anhydride 73043-80-8 >98.0%
    1-methyl-7-nitroisatoic anhydride is a reagent that detects local nucleotide flexibility, for probing 2'-hydroxyl reactivity.
    1-Methyl-7-nitroisatoic anhydride
  • HY-D0711
    Cardiogreen 3599-32-4
    Cardiogreen is fluorescent agent with low toxicity has been widely used in medical diagnostics, such as determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography.
    Cardiogreen
  • HY-66019
    FITC 3326-32-7
    FITC is a derivative of fluorescein used in wide-ranging applications including flow cytometry.
    FITC
  • HY-D0718
    Nile Red 7385-67-3 98.06%
    Nile Red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry.
    Nile Red
  • HY-D0079
    Dihydroethidium 104821-25-2 98.09%
    Dihydroethidium(Hydroethidine; PD-MY 003) is a superoxide indicator; exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red.
    Dihydroethidium
  • HY-15559A
    Hoechst 33342 trihydrochloride 875756-97-1 99.79%
    Hoechst 33342 trihydrochloride is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.
    Hoechst 33342 trihydrochloride
  • HY-14536
    Methylene Blue 61-73-4 98.22%
    Methylene blue(Basic Blue 9) is used as a dye in chromoendoscopy, and is sprayed onto the mucosa of the gastrointestinal tract in order to identify dysplasia, or pre-cancerous lesions.
    Methylene Blue
  • HY-15941
    5(6)-FITC 27072-45-3
    5(6)-FITC (Fluorescein isothiocyanate) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA.
    5(6)-FITC
  • HY-15623
    Hoechst 33258 analog 258843-62-8 99.16%
    Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA.
    Hoechst 33258 analog
  • HY-D0940
    H2DCFDA 4091-99-0 >98.0%
    H2DCFDA is a cell-permeable non-fluorescent (but fluorescein-containing) probe.
    H2DCFDA
  • HY-D0802
    Biotin NHS 35013-72-0
    Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides.
    Biotin NHS
  • HY-15937
    5(6)-FAM SE 117548-22-8 98.98%
    5(6)-FAM SE is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.
    5(6)-FAM SE
  • HY-15559
    Hoechst 33342 23491-52-3 98.75%
    Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.
    Hoechst 33342
  • HY-B1046
    Clofazimine 2030-63-9 98.09%
    Clofazimine is a fat-soluble iminophenazine dye, has a marked anti-inflammatory effect, has been used in combination with other antimycobacterial drugs to treat AIDS and Crohn's disease.
    Clofazimine
  • HY-15922
    Luminol 521-31-3 99.29%
    Luminol(Diogenes reagent) is a versatile chemical that exhibits chemiluminescence, with a striking blue glow, when mixed with an appropriate oxidizing agent.
    Luminol
  • HY-D0814
    DAPI dihydrochloride 28718-90-3 >98.0%
    DAPI dihydrochloride (4',6-diamidino-2-phenylindole) is a fluorescent stain by binding in the minor grove of A-T rich sequences of DNA.
    DAPI dihydrochloride
  • HY-19948
    Leucomethylene blue Mesylate 1236208-20-0 >98.0%
    Leucomethylene blue (Mesylate) is a common reduced form of Methylene Blue, Methylene Blue is a member of the thiazine class of dyes.
    Leucomethylene blue Mesylate