1. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.
A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy[1].
Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima[2].

References:
[1]Maes T, et al. A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile Red. Sci Rep. 2017 Mar 16;7:44501.
[2] Bruijns B, et al. Dataset of the absorption, emission and excitation spectra and fluorescence intensity graphs offluorescent cyanine dyes for the quantification of low amounts of dsDNA. Data Brief. 2016 Nov 28;10:132-143.

Dye Reagents (50):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-15534
    JC-1 3520-43-2
    JC-1, a fluorescent lipophilic carbocyanine dye, that can be used to measure mitochondrial membrane potential.
    JC-1
  • HY-15924
    MTT 298-93-1
    MTT, a yellow tetrazole, is reduced to purple formazan in living cells, and used for assessing cell metabolic activity.
    MTT
  • HY-D0913
    1-Methyl-7-nitroisatoic anhydride 73043-80-8
    1-methyl-7-nitroisatoic anhydride is a reagent that detects local nucleotide flexibility, for probing 2'-hydroxyl reactivity.
    1-Methyl-7-nitroisatoic anhydride
  • HY-D0711
    Cardiogreen 3599-32-4
    Cardiogreen is fluorescent agent with low toxicity has been widely used in medical diagnostics, such as determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography.
    Cardiogreen
  • HY-D0831
    WST-8 193149-74-5
    WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.
    WST-8
  • HY-D0852
    Sodium orthovanadate 13721-39-6
    Sodium orthovanadate is an inhibitor of protein tyrosine phosphatases, alkaline phosphatases and a number of ATPases, most likely acting as a phosphate analogue.
    Sodium orthovanadate
  • HY-15925
    NBT 298-83-9
    NBT(Nitro BT;p-Nitrotetrazolium blue) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.
    NBT
  • HY-19948
    Leucomethylene blue Mesylate 1236208-20-0
    Leucomethylene blue (Mesylate) is a common reduced form of Methylene Blue, Methylene Blue is a member of the thiazine class of dyes.
    Leucomethylene blue Mesylate
  • HY-D0819
    CY5-SE 146368-14-1
    Cy5-SE is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.
    CY5-SE
  • HY-D0821
    CY5 146368-11-8
    Cy5 is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.
    CY5
  • HY-D0150
    Thiazole Orange 107091-89-4
    Thiazole Orange is a dye for reticulocyte analysis.
    Thiazole Orange
  • HY-14536
    Methylene Blue 61-73-4
    Methylene blue(Basic Blue 9) is used as a dye in chromoendoscopy, and is sprayed onto the mucosa of the gastrointestinal tract in order to identify dysplasia, or pre-cancerous lesions.
    Methylene Blue
  • HY-D0802
    Biotin NHS 35013-72-0
    Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides.
    Biotin NHS
  • HY-D0915
    Brilliant Blue FCF 3844-45-9
    Brilliant Blue FCF has the appearance of a reddish-blue powder.
    Brilliant Blue FCF
  • HY-D0818
    CY3-YNE 1010386-62-5
    CY3-YNE is a dye for the labeling of soluble proteins, peptides, and oligonucleotides/DNA.
    CY3-YNE
  • HY-D0837
    Imidazole 288-32-4
    Imidazole is a planar 5-membered ring.
    Imidazole
  • HY-B0324A
    Crystal Violet 548-62-9
    Crystal violet is a triarylmethane dye.
    Crystal Violet
  • HY-D0825
    CY7 943298-08-6
    Cy7 dyes are widely used for labeling peptides, proteins and oligos etc.
    CY7
  • HY-D0832
    CY3-N3 1621101-43-6
    CY3-N3 is a dye for the labeling of soluble proteins, peptides, and oligonucleotides/DNA.
    CY3-N3
  • HY-D0024
    Sudan I 842-07-9
    Sudan I is an organic compound, typically classified as an azo dye.
    Sudan I