1. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (233):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-D0940
    H2DCFDA 4091-99-0 99.14%
    H2DCFDA is a cell-permeable non-fluorescent (but fluorescein-containing) probe.
    H2DCFDA
  • HY-15534
    JC-1 3520-43-2
    JC-1 is a fluorescent lipophilic carbocyanine dye, which can be used to measure mitochondrial membrane potential.
    JC-1
  • HY-12591A
    D-Luciferin 2591-17-5 98.81%
    D-luciferin is the natural substrate of luciferases that catalyze the production of light in bioluminescent insects.
    D-Luciferin
  • HY-D0913
    1-Methyl-7-nitroisatoic anhydride 73043-80-8 >98.0%
    1-methyl-7-nitroisatoic anhydride is a reagent that detects local nucleotide flexibility, for probing 2'-hydroxyl reactivity.
    1-Methyl-7-nitroisatoic anhydride
  • HY-D0718
    Nile Red 7385-67-3
    Nile Red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry.
    Nile Red
  • HY-D0711
    Cardiogreen 3599-32-4 >98.0%
    Cardiogreen is fluorescent agent with low toxicity has been widely used in medical diagnostics, such as determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography.
    Cardiogreen
  • HY-15558
    Hoechst 33258 23491-44-3 99.13%
    Hoechst 33258 is a fluorescent dyes, which can be used as a cell dye for DNA.
    Hoechst 33258
  • HY-D0814
    DAPI dihydrochloride 28718-90-3 >98.0%
    DAPI dihydrochloride (4',6-diamidino-2-phenylindole) is a fluorescent stain by binding in the minor grove of A-T rich sequences of DNA.
    DAPI dihydrochloride
  • HY-D0938
    CFSE 150347-59-4
    CFSE is a fluorescent dye which can track the cell division.
    CFSE
  • HY-66019
    FITC 3326-32-7
    FITC is a derivative of fluorescein used in wide-ranging applications including flow cytometry.
    FITC
  • HY-15559A
    Hoechst 33342 trihydrochloride 875756-97-1 99.79%
    Hoechst 33342 trihydrochloride is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.
    Hoechst 33342 trihydrochloride
  • HY-D0079
    Dihydroethidium 104821-25-2 99.77%
    Dihydroethidium(Hydroethidine; PD-MY 003) is a superoxide indicator; exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red.
    Dihydroethidium
  • HY-D0802
    Biotin NHS 35013-72-0
    Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides.
    Biotin NHS
  • HY-D0815
    Propidium Iodide 25535-16-4 >98.0%
    Propidium iodide is a red-fluorescent dye that can be used to stain cells.
    Propidium Iodide
  • HY-15928
    PNPP 4264-83-9 99.85%
    PNPP is a chromogenic substrate for acid and alkaline phosphatase in ELISA assays.
    PNPP
  • HY-D0098
    Fluorescein-5-maleimide 75350-46-8 98.00%
    Fluorescein-5-maleimide is a fluorescent thiol-reactive dye used to conjugate fluorescein to proteins (excitation: 494 nm, emission: 519 nm).
    Fluorescein-5-maleimide
  • HY-12591B
    D-Luciferin potassium salt 115144-35-9 99.20%
    D-Luciferin potassium salt is the substrate of luciferases that catalyze the production of light in bioluminescent insects.
    D-Luciferin potassium salt
  • HY-D0083
    DiI 41085-99-8 >98.0%
    DiI (DiIC18(3)) is a lipophilic membrane dye that diffuses laterally to stain the entire cell. DiI is excited to emit orange-red fluorescence. The fluorescence is weak in water but strong and photostable when incorporated into membranes.
    DiI
  • HY-D0819
    CY5-SE 146368-14-1 98.10%
    Cy5-SE is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.
    CY5-SE
  • HY-14536
    Methylene Blue 61-73-4 >98.0%
    Methylene blue(Basic Blue 9) is used as a dye in chromoendoscopy, and is sprayed onto the mucosa of the gastrointestinal tract in order to identify dysplasia, or pre-cancerous lesions.
    Methylene Blue