1. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (997):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-D0711
    Indocyanine green 3599-32-4 ≥98.0%
    Indocyanine green (Foxgreen) is a low toxicic fluorescent agent that has been widely used in medical diagnostics, such as determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography.
    Indocyanine green
  • HY-B1046
    Clofazimine 2030-63-9 99.23%
    Clofazimine is an iminophenazine dye, has a marked anti-inflammatory effect, has been used in combination with other antimycobacterial drugs to treat AIDS and Crohn's disease.
  • HY-14536
    Methylene Blue 61-73-4 ≥98.0%
    Methylene blue (Basic Blue 9) is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue is a Tau aggregation inhibitor. Methylene blue reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation.
    Methylene Blue
  • HY-D0717
    DAF-FM DA 254109-22-3 ≥98.0%
    DAF-FM DA is a reagent to detect and quantify low concentrations of nitric oxide (NO); DAF-FM fluorescence can be detected by any instrument that can detect fluorescein, including flow cytometers, microscopes, fluorescent microplate readers and fluorometers.
  • HY-19948
    Leucomethylene blue mesylate 1236208-20-0 ≥98.0%
    Leucomethylene blue (TRx0237) mesylate, an orally active second-generation tau protein aggregation inhibitor (Ki of 0.12 μM), could be used for the study of Alzheimer's Disease. Leucomethylene blue mesylate is a common reduced form of Methylene Blue, Methylene Blue is a member of the thiazine class of dyes.
    Leucomethylene blue mesylate
  • HY-111377
    Amine-PEG3-Biotin 359860-27-8 99.15%
    Amine-PEG3-Biotin is a signal amplification label containing a biotin group and a terminal primary amine group.
  • HY-D0006
    Bathophenanthroline 1662-01-7
    Bathophenanthroline is an agent used for the measurement of iron concentrations in aqueous , serum and urine samples by colorimetry.
  • HY-15907
    BBD 18378-20-6 ≥98.0%
    BBD (NSC240867) is a biochemical reagent/chromogenic reagent.
  • HY-D0516
    Solvent Blue 35 17354-14-2 98.44%
    Solvent Blue 35 (Sudan Blue II; Oil Blue 35) is a dye used for colouring alcoholic and hydrocarbon based solvents. It is used for staining triglycerides in animal tissues.
    Solvent Blue 35
  • HY-15935
    X-Gluc Dicyclohexylamine 18656-96-7 99.48%
    X-Gluc Dicyclohexylamine is used as a reagent to detect β-glucuronidase, an enzyme produced by the E.
    X-Gluc Dicyclohexylamine
  • HY-112276
    Beryllon II 51550-25-5 ≥98.0%
    Beryllon II is a widely used chromogenic reagent that is used to determine many elements, such as Mo, Mg and Co, and also used for the determination of proteins.
    Beryllon II
  • HY-D1505
    4-(N,N-Dimethylamino)azobenzene-4'-isothiocyanate 7612-98-8
    4-(N,N-Dimethylamino)azobenzene-4'-isothiocyanate is a chromophoric, hydrophobic reagent for probing membrane-buried segments of intrinsic proteins.
  • HY-15905
    ALPS 82611-85-6
    ALPS(N-Ethyl-N-sulfopropylaniline sodium salt) is a bio-chemical reagents/chromogenic reagent.
  • HY-D0828
    3,6-Dichlorotrimellitic acid 137071-78-4
    3,6-Dichlorotrimellitic acid is the key precursor that is used for preparing a variety of dichlorinated fluoresceins and rhodamines such as TET and HEX.
    3,6-Dichlorotrimellitic acid
  • HY-15903
    ADOS 82692-96-4
    ADOS(82692-96-4) is a biochemical reagent/chromogenic reagent.