1. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (447):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-15924
    Thiazolyl Blue 298-93-1 ≥98.0%
    Thiazolyl Blue (MTT) is a colorimetric agent widely used to measure cell proliferation. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells.
    Thiazolyl Blue
  • HY-12591B
    D-Luciferin potassium salt 115144-35-9 ≥98.0%
    D-Luciferin potassium salt is the substrate of luciferases that catalyze the production of light in bioluminescent insects.
    D-Luciferin potassium salt
  • HY-12591A
    D-Luciferin 2591-17-5 99.87%
    D-Luciferin is the substrate of luciferases that catalyze the production of light in bioluminescent insects.
  • HY-D0831
    WST-8 193149-74-5 ≥98.0%
    WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.
  • HY-12591
    D-Luciferin sodium salt 103404-75-7 ≥98.0%
    D-Luciferin sodium salt is the substrate of luciferases that catalyze the production of light in bioluminescent insects.
    D-Luciferin sodium salt
  • HY-15934
    X-GAL 7240-90-6 ≥98.0%
    X-GAL is a widely used chromogenic β-galactosidase substrate. β-galactosidase cleaves X-gal and produce an insoluble blue compound, which is detectable.
  • HY-112641A
    AkaLumine hydrochloride 2558205-28-8
    AkaLumine hydrochloride is a luciferin analogue, with a Km of 2.06 μM for recombinant Fluc protein.
    AkaLumine hydrochloride
  • HY-15928
    PNPP disodium 4264-83-9 ≥99.0%
    PNPP (Para-nitrophenyl phosphate) disodium is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.
    PNPP disodium
  • HY-111653
    CycLuc1 1247879-16-8
    CycLuc1 is a brain penetrant luciferase substrate.
  • HY-15925
    Nitro blue tetrazolium chloride 298-83-9 ≥98.0%
    Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.
    Nitro blue tetrazolium chloride
  • HY-P0019A
    Z-Gly-Gly-Arg-AMC acetate 2070009-61-7 99.84%
    Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP).
    Z-Gly-Gly-Arg-AMC acetate
  • HY-15902
    ABTS diammonium salt 30931-67-0 99.25%
    ABTS diammonium salt is a substrate for horseradish peroxidase (HRP) conjugate.
    ABTS diammonium salt
  • HY-P1003
    Ac-DEVD-AMC 169332-61-0 99.93%
    Ac-DEVD-AMC is the Caspase-3 substrate.
  • HY-101895
    Fluorescein di(β-D-galactopyranoside) 17817-20-8 ≥98.0%
    Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm).
    Fluorescein di(β-D-galactopyranoside)
  • HY-N0116
    Hematoxylin 517-28-2 ≥98.0%
    Hematoxylin (Natural Black 1), a naturally occurring flavonoid compound derived from the logwood tree, Haematoxylon campechianum. Hematoxylin is a nuclear stain in histology and is also a potent Aβ42 fibrillogenesis inhibitor with an IC50 of 1.6 µM.
  • HY-15930
    TMB 54827-17-7 99.83%
    TMB (BM blue) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).
  • HY-D0995
    7-Methyl-6-thioguanosine 55727-10-1 ≥99.0%
    7-Methyl-6-thioguanosine (MESG) is a chromophoric substrate which can be used for the quantitation of inorganic phosphate. 7-Methyl-6-thioguanosine is also used to determine the activity of purine nucleoside phosphorylase.
  • HY-D0994
    4-Methylumbelliferyl phosphate 3368-04-5 99.93%
    4-Methylumbelliferyl phosphate (4-MUP) is a fluorogenic substrate of alkaline phosphatases.
    4-Methylumbelliferyl phosphate
  • HY-52112
    BCDA 3252-36-6 ≥99.0%
    BCDA (5-bromo-4-chloroindoxyl acetate) is a chromogenic substrate of esterase used to potently detect the activity of esterase.
  • HY-D0935A
    4-Methylumbelliferyl-β-D-glucuronide hydrate 881005-91-0 99.98%
    4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm , λem=445 nm).
    4-Methylumbelliferyl-β-D-glucuronide hydrate