1. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (271):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-12591A
    D-Luciferin 2591-17-5 98.81%
    D-luciferin is the natural substrate of luciferases that catalyze the production of light in bioluminescent insects.
    D-Luciferin
  • HY-12591B
    D-Luciferin potassium salt 115144-35-9 99.20%
    D-Luciferin potassium salt is the substrate of luciferases that catalyze the production of light in bioluminescent insects.
    D-Luciferin potassium salt
  • HY-15928
    PNPP 4264-83-9 99.85%
    PNPP is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.
    PNPP
  • HY-15925
    Nitro blue tetrazolium chloride 298-83-9 >99.0%
    Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.
    Nitro blue tetrazolium chloride
  • HY-12591
    D-Luciferin sodium salt 103404-75-7 99.91%
    D-Luciferin sodium salt is the substrate of luciferases that catalyze the production of light in bioluminescent insects.
    D-Luciferin sodium salt
  • HY-P0019A
    Z-Gly-Gly-Arg-AMC acetate 2070009-61-7 99.84%
    Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP).
    Z-Gly-Gly-Arg-AMC acetate
  • HY-P1003
    Ac-DEVD-AMC 169332-61-0 99.93%
    Ac-DEVD-AMC is the Caspase-3 substrate.
    Ac-DEVD-AMC
  • HY-112641A
    TokeOni
    TokeOni (AkaLumine hydrochloride) is a luciferin analogue, with a Km of 2.06 μM for recombinant Fluc protein.
    TokeOni
  • HY-D0055
    3-Cyano-7-ethoxycoumarin 117620-77-6 >98.0%
    3-Cyano-7-ethoxycoumarin is a fluorogenic cytochrome P-450 substrate that generates blue fluorescent product upon enzyme cleavage Target: Cytochrome P450 3-Cyano-7-ethoxycoumarin is a fluorescent probe useful in microsomal dealkylase studies.
    3-Cyano-7-ethoxycoumarin
  • HY-52112
    BCDA 3252-36-6 99.50%
    BCDA is a chromogenic substrate of esterase used to detect the activity of esterase.
    BCDA
  • HY-D0995
    MESG 55727-10-1 >99.0%
    MESG is a chromophoric substrate which can be used for the quantitation of inorganic phosphate.
    MESG
  • HY-15930
    TMB 54827-17-7 99.98%
    TMB (BM blue) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).
    TMB
  • HY-15930A
    TMB dihydrochloride 64285-73-0 99.83%
    TMB dihydrochloride (BM blue dihydrochloride) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).
    TMB dihydrochloride
  • HY-16710
    GPDA 65096-46-0 99.99%
    GPDA(Glycylproline p-nitroanilide tosylate) is the substarate of X-Prolyl dipeptidyl-aminopeptidase in the enzyme assay.
    GPDA
  • HY-111653
    CycLuc1 1247879-16-8
    CycLuc1 is a brain penetrant luciferase substrate.
    CycLuc1
  • HY-P1448
    Ac-Leu-Arg-AMC 929621-79-4 99.59%
    Ac-Leu-Arg-AMC is a fluorogenic peptide substrate.
    Ac-Leu-Arg-AMC
  • HY-D0935A
    4-Methylumbelliferyl-β-D-glucuronide hydrate 881005-91-0 99.98%
    4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm , λem=445 nm).
    4-Methylumbelliferyl-β-D-glucuronide hydrate
  • HY-15909
    BCIP 6578-06-9 98.85%
    BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity.
    BCIP
  • HY-D0994
    4-Methylumbelliferyl phosphate 3368-04-5 99.93%
    4-methylumbelliferyl phosphate is a fluorogenic substrate of phosphatases.
    4-Methylumbelliferyl phosphate
  • HY-15930C
    TMB monosulfate 54827-18-8 99.82%
    TMB monosulfate is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualizing reagent used in enzyme-linked immunosorbent assays (ELISA).
    TMB monosulfate