2. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (1066):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-15559
    Hoechst 33342 23491-52-3 ≥98.0%
    Hoechst 33342 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. Storage: Keep away from light.
    Hoechst 33342
  • HY-15559A
    Hoechst 33342 trihydrochloride 875756-97-1 99.81%
    Hoechst 33342 trihydrochloride is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. Storage: Keep away from light.
    Hoechst 33342 trihydrochloride
  • HY-15558A
    Hoechst 33258 trihydrochloride 23491-45-4 98.87%
    Hoechst 33258 trihydrochloride is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. Storage: Keep away from light.
    Hoechst 33258 trihydrochloride
  • HY-15560B
    Hoechst 34580 tetrahydrochloride 99.77%
    Hoechst 34580 tetrahydrochloride is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. Storage: Keep away from light.
    Hoechst 34580 tetrahydrochloride
  • HY-D1020
    7-Aminoactinomycin D 7240-37-1 ≥99.0%
    7-Aminoactinomycin D (7-AAD) a fluorescent DNA stain, is a potent RNA polymerase inhibitor. 7-Aminoactinomycin D selectively binds to GC regions of the DNA. 7-Aminoactinomycin D also has antibacterial effects.
    7-Aminoactinomycin D
  • HY-D0996
    LDS-751 181885-68-7 ≥99.0%
    Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 has a high affinity for DNA and fluorescence is enhanced after binding, but the maximum emission wavelength is 670nm. Lds-751 and Thiazole orange can be used for the differentiation of red blood cells, platelets, reticulocytes, and nucleated cells and can be stimulated at 488nm. Studies have shown that LDS-751 binds almost exclusively to mitochondria when incubated with nucleated living cells. After nucleated Acridine Orange (HY-101879) staining and LDS-751 treatment of cells, confocal microscopy revealed almost no co-location of the cells. Staining with Rhodamine 123 (HY-D0816), a dye known to bind polarized mitochondria, was almost identical to the pattern observed with LDS-751. Storage: Keep away from light.
    LDS-751
  • HY-133523
    HBC514 2530161-67-0 ≥98.0%
    HBC514 is a nonfluorescent HBC-analog but emits strong green fluorescence upon forming a tight complex with Pepper RNA aptamer. HBC514-Pepper complex enables visualization of RNAs and the fluorescences can be altered flexibly by simple washing and staining in living Pepper-tagged cells[1]. Storage: protect from light.
    HBC514
  • HY-108166A
    Hydroxystilbamidine bis(fluoroGlod) 223769-64-0
    Hydroxystilbamidine bis(methanesulfonate), a dye capable of binding to both DNA and RNA, has been found to be a powerful inhibitor of cellular ribonucleases. Storage: protect from light.
    Hydroxystilbamidine bis(fluoroGlod)
  • HY-15623
    Hoechst 33258 analog 258843-62-8 99.96%
    Hoechst 33258 analog is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. Storage: Keep away from light.
    Hoechst 33258 analog
  • HY-15626
    ortho-iodoHoechst 33258 158013-41-3 98.16%
    ortho-iodoHoechst 33258 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. Storage: Keep away from light.
    ortho-iodoHoechst 33258
  • HY-D0093
    Ethidium homodimer 61926-22-5 ≥99.0%
    Ethidium homodimer (EthD-1) is a cell-membrane impermeant nucleic acid fluorochrome with high affinity for DNA. Ethidium homodimer can be used to test cell viability. Storage: protect from light.
    Ethidium homodimer
  • HY-15630A
    Hoechst 33342 analog 2 trihydrochloride 155815-98-8 98.02%
    Hoechst 33342 analog 2 trihydrochloride is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. Storage: Keep away from light.
    Hoechst 33342 analog 2 trihydrochloride
  • HY-D0950A
    Methyl Green zinc chloride 7114-03-6
    Methyl Green zinc chloride is a potent fluorescent dye. Methyl Green zinc chloride is a DNA stains of cells and electrophoretic gels. Methyl Green zinc chloride can be used as direct measuring of viability by both microscopy and flow cytometry, with peaks at 633 and 677 nm. Storage: protect from light.
    Methyl Green zinc chloride
  • HY-15631
    Hoechst 33258 analog 6 129244-66-2 99.63%
    Hoechst 33258 analog 6 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. Storage: Keep away from light.
    Hoechst 33258 analog 6
  • HY-15558
    Hoechst 33258 23491-44-3 ≥98.0%
    Hoechst 33258 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. Storage: Keep away from light.
    Hoechst 33258
  • HY-D0163
    Methyl Green 14855-76-6
    Methyl Green is a potent fluorescent dye. Methyl Green is a DNA stains of cells and electrophoretic gels. Methyl Green can be used as a stain for direct measuring of viability by both microscopy and flow cytometry, with peaks at 633 and 677 nm. Storage: protect from light.
    Methyl Green
  • HY-15627
    Hoechst 33342 analog 178481-68-0 98.04%
    Hoechst 33342 analog is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. Storage: Keep away from light.
    Hoechst 33342 analog
  • HY-15632
    para-iodoHoechst 33258 158013-43-5 99.73%
    para-iodoHoechst 33258 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. Storage: Keep away from light.
    para-iodoHoechst 33258
  • HY-15619
    Hoechst S 769121 74681-68-8
    Hoechst S 769121 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. Storage: Keep away from light.
    Hoechst S 769121
  • HY-D0917
    TO-PRO 1 157199-59-2
    TO-PRO 1 is a DNA binding fluorochrome, that atached to the surface of the Feraheme (FH) nanoparticle (NP), to obtain a fluorochrome-functionalized NP. TO-PRO 1 binds DNA through intercalation, and acts as a vital fluorochrome for necrotic cells. Storage: protect from light.
    TO-PRO 1