1. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (410):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-15559A
    Hoechst 33342 trihydrochloride 875756-97-1 99.87%
    Hoechst 33342 trihydrochloride is a membrane permeant blue fluorescent DNA stain.
    Hoechst 33342 trihydrochloride
  • HY-15558A
    Hoechst 33258 trihydrochloride 23491-45-4 99.71%
    Hoechst 33258 trihydrochloride is a fluorescent dyes, which can be used as a cell dye for DNA.
    Hoechst 33258 trihydrochloride
  • HY-D1020
    7-Aminoactinomycin D 7240-37-1
    7-Aminoactinomycin D (7-AAD) a fluorescent DNA stain, is a potent RNA polymerase inhibitor. 7-Aminoactinomycin D selectively binds to GC regions of the DNA. 7-Aminoactinomycin D also has antibacterial effects.
    7-Aminoactinomycin D
  • HY-D0996
    LDS-751 181885-68-7 ≥99.0%
    LDS-751 is a nucleic acid stain principally detecting DNA. LDS-751 has high affinity for DNA and undergoes fluorescence enhancement upon binding, but with maximal emission at 670 nm. LDS-751 and Thiazole orange are used to differentiate erythrocytes, platelets, reticulocytes, and nucleated cells, and both can be excited at 488 nm.
    LDS-751
  • HY-15623
    Hoechst 33258 analog 258843-62-8 99.96%
    Hoechst 33258 analog are part of a family of blue fluorescent dyes used to stain DNA.
    Hoechst 33258 analog
  • HY-15560B
    Hoechst 34580 tetrahydrochloride 99.58%
    Hoechst 34580 tetrahydrochloride is a cell-permeable fluorescent dye for staining DNA and nuclei.
    Hoechst 34580 tetrahydrochloride
  • HY-15559
    Hoechst 33342 23491-52-3 99.24%
    Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.
    Hoechst 33342
  • HY-15558
    Hoechst 33258 23491-44-3 ≥98.0%
    Hoechst 33258 is a fluorescent dye that emits blue fluorescence when bound to dsDNA.
    Hoechst 33258
  • HY-133523
    HBC514 ≥98.0%
    HBC514 is a nonfluorescent HBC-analog but emits strong green fluorescence upon forming a tight complex with Pepper RNA aptamer. HBC514-Pepper complex enables visualization of RNAs and the fluorescences can be altered flexibly by simple washing and staining in living Pepper-tagged cells.
    HBC514
  • HY-15630A
    Hoechst 33342 analog 2 trihydrochloride 155815-98-8 98.13%
    Hoechst 33342 analog 2 trihydrochloride is a anglog of Hoechst 33342. Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.
    Hoechst 33342 analog 2 trihydrochloride
  • HY-108166A
    Hydroxystilbamidine bis(methanesulfonate) 223769-64-0
    Hydroxystilbamidine bis(methanesulfonate), a dye capable of binding to both DNA and RNA, has been found to be a powerful inhibitor of cellular ribonucleases.
    Hydroxystilbamidine bis(methanesulfonate)
  • HY-15621A
    DMA trihydrochloride 2095832-33-8 99.24%
    DMA trihydrochloride is a fluorescent compound (λex=340 nm, λem=478 nm).
    DMA trihydrochloride
  • HY-15632
    para-iodoHoechst 33258 158013-43-5 99.73%
    para-iodoHoechst 33258 is part of a family of blue fluorescent dyes used to stain DNA.
    para-iodoHoechst 33258
  • HY-15621
    DMA 188860-26-6 99.64%
    DMA is a fluorescent compound (λex=340 nm, λem=478 nm).
    DMA
  • HY-15619
    Hoechst S 769121 74681-68-8
    Hoechst S 769121 (Nuclear yellow) exhibits excitation/emission maxima ~335/495 nm when bound to DNA.
    Hoechst S 769121
  • HY-15562
    HOE 32021 23623-06-5 99.95%
    Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA.
    HOE 32021
  • HY-15561
    HOE-S 785026 132869-83-1 99.12%
    HOE-S 785026 is a blue fluorescent dyes, which can be used as a cell dye for DNA.
    HOE-S 785026
  • HY-15560
    Hoechst 34580 23555-00-2 99.84%
    Hoechst 34580 is a cell-permeable fluorescent dye for staining DNA and nuclei.
    Hoechst 34580
  • HY-15626
    ortho-iodoHoechst 33258 158013-41-3 98.16%
    ortho-iodoHoechst 33258 is part of a family of blue fluorescent dyes used to stain DNA.
    ortho-iodoHoechst 33258
  • HY-15627
    Hoechst 33342 analog 178481-68-0 98.04%
    Hoechst 33342 analog is an anglog of Hoechst 33342, which is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.
    Hoechst 33342 analog