1. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (316):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-D0940
    H2DCFDA 4091-99-0 99.82%
    H2DCFDA is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS).
  • HY-15534
    JC-1 3520-43-2 99.46%
    JC-1 is a fluorescent lipophilic carbocyanine dye used to measure mitochondrial membrane potential.
  • HY-D0938
    CFSE 150347-59-4
    CFSE is a fluorescent dye which can track the cell division.
  • HY-D0814
    DAPI dihydrochloride 28718-90-3 >98.0%
    DAPI dihydrochloride (4',6-diamidino-2-phenylindole) is a fluorescent stain by binding in the minor grove of A-T rich sequences of DNA.
    DAPI dihydrochloride
  • HY-D0718
    Nile Red 7385-67-3
    Nile Red is a strongly fluorescent stain in the presence of a hydrophobic environment for the detection of intracellular lipid droplets.
    Nile Red
  • HY-D0041
    Calcein-AM 148504-34-1 >98.0%
    Calcein-AM is cell-permeable fluorescent dye used to determine the cell viability.
  • HY-D0815
    Propidium Iodide 25535-16-4 >98.0%
    Propidium Iodide is a red-fluorescent dye that can be used to stain cells.
    Propidium Iodide
  • HY-111382
    Diphenylterazine 344940-63-2 98.06%
    Diphenylterazine is a bioluminescence agent.
  • HY-66019
    FITC 3326-32-7 >98.0%
    FITC is a derivative of fluorescein for the labeling of amines.
  • HY-D0985A
    TMRE 115532-52-0
    TMRE is a mitochondria specific dye (λex=550 nm, λem=575 nm).
  • HY-D0083
    DiI 41085-99-8 99.92%
    DiI (DiIC18(3)) is a lipophilic membrane dye that diffuses laterally to stain the entire cell. DiI is excited to emit orange-red fluorescence. The fluorescence is weak in water but strong and photostable when incorporated into membranes.
  • HY-D0079
    Dihydroethidium 104821-25-2 99.77%
    Dihydroethidium(Hydroethidine; PD-MY 003) is a superoxide indicator; exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red.
  • HY-D0098
    Fluorescein-5-maleimide 75350-46-8 98.00%
    Fluorescein-5-maleimide is a fluorescent thiol-reactive dye used to conjugate fluorescein to proteins (excitation: 494 nm, emission: 519 nm).
  • HY-D0090
    MQAE 162558-52-3 99.84%
    MQAE is a fluorescent indicator that is quenched via collision with chloride, and is more sensitive and selective than 36Cl and microelectrode-based methods for chloride measurement in cells.
  • HY-D0816
    Rhodamine 123 62669-70-9 98.17%
    Rhodamine 123 (RH-123; R-22420) is a fluorescent dye (λex=503 nm, λem=527 nm).
    Rhodamine 123
  • HY-110250
    DFHBI 1241390-29-3 99.11%
    DFHBI is a small molecule that resembles the chromophore of green fluorescent protein (GFP). Spinach and DFHBI are essentially nonfluorescent when unbound, whereas the Spinach-DFHBI complex is brightly fluorescent both in vitro and in living cells.
  • HY-128868
    FITC-Dextran 60842-46-8
    FITC-Dextran is a marker consisting of coupling fluorescein-isothiocyanate to dextran (10 kDa). FITC-Dextrans are polysaccharides comprised of varying lengths of branched glucose molecules and can be used to determine solute, ion and protein permeability of the blood-brain barrier (BBB) based on the size of the dextran used.
  • HY-101883
    BCECF-AM 117464-70-7 >99.0%
    BCECF-AM is a cell membrane permeable compound, widely used as a fluorescent indicator for intracellular pH.
  • HY-D0150
    Thiazole Orange 107091-89-4 99.84%
    Thiazole Orange is a dye for reticulocyte analysis.
    Thiazole Orange
  • HY-101879
    Acridine Orange hydrochloride 65-61-2 99.86%
    Acridine Orange hydrochloride is a cell-permeable fluorescent dye that binds to nucleic acids, resulting in an altered spectral emission.
    Acridine Orange hydrochloride