1. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (437):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-D0819
    CY5-SE 146368-14-1 ≥98.0%
    Cy5-SE (Cy5 NHS Ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. Excitation (nm):649, Emission (nm): 670.
    CY5-SE
  • HY-D0826
    Cy2-SE (iodine) 186205-33-4
    CY2-SE (Cyanine2 Succinimidyl Ester) is a dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.
    Cy2-SE (iodine)
  • HY-D0821
    CY5 146368-11-8 98.81%
    Cy5 (Sulfo-Cyanine5) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. Excitation (nm):649, Emission (nm): 670.
    CY5
  • HY-D0822
    CY3 146368-13-0 ≥98.0%
    Cy3 (Sulfo-Cyanine3) is an orange-fluorescent label for protein and nucleic acid (λex=554, λem=568).
    CY3
  • HY-101890
    Dabsyl chloride 56512-49-3 ≥98.0%
    Dabsyl chloride is an amine derivatizing agent, able to give rise to stable products that can be easily monitored spectrophotometrically at 460 nm; Dabsyl chloride also used for labeling amino acids.
    Dabsyl chloride
  • HY-D0017
    Dansyl chloride 605-65-2 ≥98.0%
    Dansyl chloride is a reagent that reacts with primary amino groups in both aliphatic and aromatic amines to produce stable blue- or blue-green-fluorescent sulfonamide adducts.
    Dansyl chloride
  • HY-D0832
    CY5-N3 1621101-43-6 98.40%
    CY5-N3 is a Cy5-azide, which is a fluorescent dye.
    CY5-N3
  • HY-D0715
    Fluorescamine 38183-12-9 ≥98.0%
    Fluorescamine( Ro 20-7234) is a spiro compound that is not fluorescent itself, but reacts with primary amines to form highly fluorescent products.
    Fluorescamine
  • HY-D0827
    Cy2 260430-02-2 98.07%
    CY2 Non-Sulfonated (Cyanine2) is a dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.
    Cy2
  • HY-D0820
    CY5-YNE 1345823-20-2
    CY5-YNE (Sulfo-Cyanine5-alkyne) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.
    CY5-YNE
  • HY-D0818
    CY3-YNE 1010386-62-5
    CY3-YNE (Sulfo-Cyanine3-alkyne) is a dye for the labeling of soluble proteins, peptides, and oligonucleotides/DNA.
    CY3-YNE
  • HY-D0968
    Cy 3 Non-Sulfonated 146397-20-8
    Cy 3 Non-Sulfonated (Cyanine3) is a fluorescent label for protein and nucleic acid.
    Cy 3 Non-Sulfonated
  • HY-125750
    Coumarin 7 27425-55-4
    Coumarin 7 is a coumarin laser dye in plants in the form of glycosides.
    Coumarin 7