MCE Kits

RNase Inhibitor is a 50 kDa recombinant protein expressed in E.coli, and can specifically inhibit RNase A, B and C.

MCE RT Master Mix for qPCR II is a convenient, ready-to-use formulation for reverse transcription and can eliminate genomic DNA (gDNA) contaminations in RNA samples. With updated reverse transcriptase, this kit can synthesize cDNA more rapidly, more specifically.

MCE Agarose With TAE Powder (1%) mainly consists of proportionally mixed agarose and TAE Powder, and can be directly made into 1% agarose gel.

MCE Agarose can be made into 0.5-2.5% agarose gel according to different needs and can resolve DNA and RNA fragments from 50-15,000 bp.

MCE TAE Powder (1 L of 1×) consists of Tris-acetate and EDTA-2Na, and is widely used in agarose gel electrophoresis of nucleic acid.

MCE TBE Powder (1 L of 1×) consists of Tris, Boric Acid and EDTA, and is widely used in agarose gel electrophoresis of nucleic acid.

MCE Rapid Running Buffer Powder (1 L of 1×) is mainly used for agarose gel electrophoresis of nucleic acid.

MCE 6× DNA Loading Buffer with SDS is a modified 6-fold buffer and mainly consists of glycerin, EDTA, SDS, Orange G and Xylene Cyanol FF.

MCE Seamless DNA Assembly Plus Kit contains an optimized mix of recombinase, reaction buffer, and additional cofactors that significantly improve the cloning efficiency and tolerance to impurities. This product can complete multiple DNA fragments recombination and takes only 5 minutes for single fragment, and the positive rate is more than 95%.

MCE Genomic DNA Mini Purification Kit allows rapid and efficient purification of genomic DNA based on the selective binding of DNA to the silica-based membrane. Genomic DNA purified with this kit can be used for PCR, qPCR, enzyme digestion, Southern Blot, etc.

MCE Viral DNA/RNA Mini Purification Kit allows rapid and efficient purification of viral DNA/RNA based on the selective binding of DNA/RNA to the silica-based membrane. Viral DNA/RNA purified with this kit can be used for PCR, RT-PCR, qPCR, and qRT-PCR.

MCE Plasmid DNA Maxi Purification Kit allows rapid and efficient purification of plasmid DNA with the time-tested consistency of alkaline-SDS lysis of bacterial cells.

MCE PBS Powder (1 L of 1×) consists of NaCl, KCl and Phosphate, and is widely used in immunoassay, microbiology and protein experiments.

MCE PBS Powder (2 L of 1×) consists of NaCl, KCl and Phosphate, and is widely used in immunoassay, microbiology and protein experiments.

MCE TBS Powder (1 L of 1×) consists of NaCl, KCl and Tris, and is widely used as washing buffer in immunocytochemistry, FISH or ELISA, and can also be used for antibody dilution and preparation of blocking solution.

MCE Protease Inhibitor Cocktail, Bacteria (EDTA-Free, 100× in DMSO) protects proteins from degradation by endogenous proteases released during protein extraction and purification.

MCE Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O) protects protein from degradation by endogenous proteases released during protein extraction and purification.

MCE Kinase Inhibitor Cocktail (5×, in ddH₂O) controls the biological activity of kinases. This small molecule/inhibitor is primarily used for Biochemicals applications.

MCE Anti-HA Magnetic Beads (1 μm) are used for immunoprecipitation (IP) of specific HA-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

MCE Anti-c-Myc Magnetic Beads (1 μm) are used for immunoprecipitation (IP) of specific c-Myc-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

MCE Anti-Flag Magnetic Beads are used for immunoprecipitation (IP) of specific Flag-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

MCE Anti-His Magnetic Beads are used for immunoprecipitation (IP) of specific His-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

MCE Anti-Flag Affinity Gel is used for immunoprecipitation (IP) or protein purification of specific Flag-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

MCE Streptavidin Agarose 6FF, a 6% highly cross-linked agarose reagent coupled with recombinant streptavidin, is an affinity chromatography medium for separation and purification of biotinylated peptides, antibodies, lectins, etc. The total binding capacity of Streptavidin Agarose 6FF is more than 200 nmol of D-Biotin/mL settled resin.

MCE High-Affinity Iodoacetyl Agarose, a 4% highly cross-linked agarose reagent coupled with a derivative of iodoacetic acid, is ideal for conjugating sulfhydryl-containing peptide or protein for subsequent affinity purification.

MCE Ni-NTA 6FF Prepacked Column is ideal for high performance purification of polyhistidine-tagged proteins expressed in E.coli, yeast, insect and mammalian expression systems.

MCE PBS Powder (1 L of 1×) consists of NaCl, KCl and Phosphate, and is widely used in immunoassay, microbiology and protein experiments.

MCE PBS Powder (2 L of 1×) consists of NaCl, KCl and Phosphate, and is widely used in immunoassay, microbiology and protein experiments.

MCE PBS-T Powder (1 L of 1×) consists of NaCl, KCl, Phosphate and Tween 20, and is widely used in ELISA and Western Blot, and can also be used for antibody dilution and preparation of blocking solution.

MCE TBS-T Powder (1 L of 1×) consists of NaCl, KCl, Tris and Tween 20, and is widely used as washing buffer in immunocytochemistry, FISH or ELISA, and can also be used for antibody dilution and preparation of blocking solution.

MCE Tris-MES-SDS Powder (1 L of 1×) consists of Tris, MES, EDTA and SDS, and is mainly used for separation of small- to medium-sized proteins.

MCE Tris-MOPS-SDS Powder (1 L of 1×) consists of Tris, MOPS, EDTA and SDS, and is mainly used for separation of medium- to large-sized proteins.

MCE Tris-Glycine-SDS Powder (1 L of 1×) consists of Tris, Glycine and SDS, and is widely used as an electrophoresis buffer for SDS-PAGE and Western Blot.

MCE SDS dust-free Powder can be directly prepared into 10% SDS solution, and is widely used for protein denaturation.

MCE Tris-HEPES-SDS Powder (500 mL of 1×) consists of Tris, HEPES, EDTA and SDS, and is widely used in protein electrophoresis.

MCE Tris-Glycine Powder (1 L of 1×) consists of Tris and Glycine, and is widely used as an electrophoresis buffer for Native-PAGE.

MCE Transfer Buffer (Semi Dry) Powder (1 L of 1×) consists of Tris, Glycine and SDS, and is suitable for Western Blot semi-dry electrophoresis.

MCE Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×) mainly consists of fish gelatin protein, and is widely used for the antibody blocking step in Western Blot or ELISA within 15 minutes.

MCE PBS-T Powder (1 L of 1×) consists of NaCl, KCl, Phosphate and Tween 20, and is widely used in ELISA and Western Blot, and can also be used for antibody dilution and preparation of blocking solution.

MCE TBS-T Powder (1 L of 1×) consists of NaCl, KCl, Tris and Tween 20, and is widely used as washing buffer in immunocytochemistry, FISH or ELISA, and can also be used for antibody dilution and preparation of blocking solution.

SDS-PAGE Sample Loading Buffer (4×) is appropriate for loading protein samples on SDS-PAGE.

MCE PEI Transfection Reagent is designed based on 25 kDa PEI. It has high-efficiency, low-toxicity, strong-stability, and is suitable for many cell types, such as HEK-293、HEK-293T、CHO-K1、COS-1、COS-7、NIH/3T3、Sf9、HepG2 and HeLa et, even some hard-to-transfect cells. It can also be applied to large-scale recombinant protein expression and virus production.

MCE BM-Cyclin mainly contains Tiamulin fumarate and Minocycline hydrochloride, can effectively inhibit and remove mycoplasma contamination widespread in cell cultures. It is also effective against some common bacteria.

MCE Gentamicin, Sterile acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria, mainly for gram-negative and gram-positive bacteria.

MCE Hygromycin B, Sterile is an aminoglycosidic antibiotic purified from Streptomyces hygroscopicus. It acts by binding to the 70S subunit of the bacterial ribosome and inhibiting protein synthesis, leading to the death of bacteria, fungi and mammalian cells.

MCE Amphotericin B is a polyene antifungal antibiotic produced by Streptomyces nodosus. It can bind with sterols to form transmembrane channels, resulting in the leakage of intracellular substances, thus inhibiting fungal and yeast contamination.

MCE Zeocin Selection Antibiotic, Sterile is a formulation of phleomycin D1, produced by Streptomyces verticillus, is an alkaline, water-soluble, copper ion chelated glycopeptide antibiotic.

MCE Blasticidin S, Sterile is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation.

MCE Kanamycin sulfate (100x), Sterile is an aminoglycoside antibiotic purified from Streptomyces kanamyceticus. It acts by binding to the 30S subunit of the bacterial ribosome and inhibiting protein synthesis in susceptible bacteria.

MCE G418 Selective Antibiotic, Sterile is an aminoglycoside antibiotic produced by Micromonospora rhodorangea. It acts by binding to the 80S subunit of the bacterial ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells.

MCE Puromycin, Sterile is an aminonucleoside antibiotic produced by Streptomyces alboniger. It inhibits protein synthesis by disrupting peptide transfer on ribosomes, causing premature chain termination during translation. It can kill most gram-positive bacteria and various animal or insect cells.

MCE Antibiotic-Antifungal (100 ×), Sterile contains 10 KU/mL of penicillin, 10 mg/mL of streptomycin, and 25 μg/mL of Amphotericin B. The antibiotics penicillin and streptomycin prevent bacterial contamination, and Amphotericin B prevents fungal contamination.

MCE CEPT Cocktail is composed of CET small molecules (Chroman 1, Emricasan and Trans-ISRIB) and Polyamine Solution, and can effectively enhance cell survival and promote clonal growth of genetically stable hPSCs.

MEM Non-essential Amino Acid Solution (100×) has been filtered and can be used directly. It adds nutrients and reduces the biosynthetic burden on cell in vitro.

Fetal Bovine Serum (FBS) is derived from healthy bovine in epidemic-free areas, prepared from the blood of 5-8-month-old fetal bovine, origin is Uruguay.

MCE CTG Cell Viability Detection Reagent is used for detecting the number and viability of living cells in culture based on high-sensitivity bioluminescence detection technology of the ATP present.

MCE Cytotoxicity LDH Assay Kit can detect LDH activity in the culture supernatant, which is indicative of cytotoxicity. Meanwhile viable cell number can be detected by CCK8 Kit (HY-K0301).

MCE Apoptosis and Necrosis Assay Kit provides a rapid and convenient method to detect cell apoptosis and necrosis.

MCE Cell Cycle and Apoptosis Analysis Kit (PI staining) provides a convenient method to detect cell cycle and cell apoptosis.

MCE Cell Apoptosis Analysis Kit (Hoechst staining) provides a rapid and convenient method to detect cell apoptosis.

MCE Annexin V-FITC/PI Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence (Annexin V-/PI-), early apoptosis cells show green fluorescence(Annexin V+/PI-), late apoptosis cells and necrosis cells show red and green fluorescence (Annexin V+/PI+).

Annexin V-EGFP/PI Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence (Annexin V-/PI-), early apoptosis cells show green fluorescence(Annexin V+/PI-), late apoptosis cells and necrosis cells show red and green fluorescence (Annexin V+/PI+). Compared with FITC, the fluorescence of EGFP is stronger and more stable.

MCE Annexin V-PE Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence, apoptosis cells and necrosis cells show red fluorescence.

Annexin V-mCherry Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence, apoptosis cells and necrosis cells show red fluorescence.

Annexin V-mCherry/SYTOX Green Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence, apoptosis cells show red fluorescence, necrosis cells show red and green fluorescence.

MCE One Step TUNEL Apoptosis Detection Kit (FITC) provides a rapid and convenient method to detect cell apoptosis. After staining cells with this kit, live cells have no fluorescence, apoptosis cells show green fluorescence.

MCE One Step TUNEL Apoptosis Detection Kit (Cyanine 3) provides a rapid and convenient method to detect cell apoptosis. After staining cells with this kit, live cells have no fluorescence, apoptosis cells show red fluorescence.

MCE Annexin V-iFluor 488/PI Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence (Annexin V-/PI-),, early apoptosis cells show green fluorescence (Annexin V+/PI-), late apoptosis cells and necrosis cells show red and green fluorescence (Annexin V+/PI+).

MCE AntiFade Mounting Medium can slow the fluorescence quenching of various common fluorescent dyes with simple operation and good anti-fluorescence quenching effect.

MCE Mitochondria Isolation Kit for Cultured Cells enables the fast and efficient isolation of intact mitochondria from cells using differential centrifugation.

MCE Mitochondria Isolation Kit for Tissue enables the fast and efficient isolation of intact mitochondria from tissue using differential centrifugation.

MCE Exosome Isolation and Purification Kit provides a simple and effective method to isolate and purify intact exosomes from cell culture media that can be used for electron microscope analysis, NTA analysis, WB, qPCR, etc.

MCE Exosome Isolation and Purification Kit provides a simple and effective method to isolate and purify intact exosomes from plasma and serum that can be used for electron microscope analysis, NTA analysis, WB, qPCR, etc.

MCE Exosome Protein Detection Kit (CD63&TSG101) can specifically detect exosome proteins CD63 and TSG101. This product is suitable for the detection of human, rat and mouse exosomes.

MCE Exosome Isolation and Purification Kit provides a simple and effective method to isolate and purify intact exosomes from cerebro-spinal fluid, amniotic fluid, milk, saliva, etc, which can be used for electron microscope analysis, NTA analysis, WB, qPCR, etc.