1. Academic Validation
  2. Optimum dose of LH-RH analogue Fertirelin Acetate for the induction of superovulation in mice

Optimum dose of LH-RH analogue Fertirelin Acetate for the induction of superovulation in mice

  • Exp Anim. 2005 Jan;54(1):97-9. doi: 10.1538/expanim.54.97.
Koichi Nariai 1 Tsuyoshi Ishinazaka Kazuyosi Suzuki Hiroshi Uchiyama Kahei Sato Ryuji Asano Shigehisa Tsumagari Masayoshi Yukawa Kiichi Kanayama
Affiliations

Affiliation

  • 1 Laboratory Animal Facilities, Research Center for Medical Sciences, The Jikei University School of Medicine, Kashiwa, Chiba, Japan.
Abstract

The optimum dose for establishing superovulation in mice of Fertirelin Acetate (FA), an LH-RH analogue, was examined. Mice were subcutaneously injected with 5 IU of hCG at 17:00 (Day 0), and with various doses of FA (0.001 to 1.0 microg) five times at 4 h intervals on and after 22:00 on Day 0. To induce ovulation, 5 IU of hCG was again injected subcutaneously at 17:00 on Day 2. In the groups administered with doses ranging from 0.01 to 0.5 microg of FA, the number of ovulated eggs was significantly (p<0.05) larger than in the control group (12.9 +/- 5.9). The greatest number of ovulated eggs (22.6 +/- 7.3) was obtained in the group administered with 0.025 microg of FA. The results indicate that the effective dose of LH-RH analogue, FA, is between 0.1 and 0.5 microg for superovulation induction in mice.

Figures