2. Academic Validation
  3. Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines

Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines

  • Cancer Lett. 2012 Feb 1;315(1):1-11. doi: 10.1016/j.canlet.2011.09.042.
Chun-Han Chen 1,  Cho-Hwa Liao,  Ya-Ling Chang,  Jih-Hwa Guh,  Shiow-Lin Pan,  Che-Ming Teng
Affiliations

Affiliation

  • 1 Pharmacological Institute, College of Medicine, National Taiwan University, Taipei, ROC.
PMID: 22033245 DOI: 10.1016/j.canlet.2011.09.042
Abstract

In this study, we investigated the Anticancer effect of protopine on human hormone-refractory prostate Cancer (HRPC) cells. Protopine exhibited an anti-proliferative effect by induction of tubulin polymerization and mitotic arrest, which ultimately led to apoptotic cell death. The data suggest that protopine increased the activity of cyclin-dependent kinase 1 (CDK1)/cyclin B1 complex and that contributed to cell Apoptosis by modulating mitochondria-mediated signaling pathways, such as Bcl-2 phosphorylation and Mcl-1 down-regulation. In conclusion, the data suggest that protopine is a novel microtubule stabilizer with Anticancer activity in HRPC cells through apoptotic pathway by modulating CDK1 activity and Bcl-2 Family of proteins.

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