2. Academic Validation
  3. Enhancement of IGF-2-induced neurite outgrowth by IGF-binding protein-2 and osteoglycin in SH-SY5Y human neuroblastoma cells

Enhancement of IGF-2-induced neurite outgrowth by IGF-binding protein-2 and osteoglycin in SH-SY5Y human neuroblastoma cells

  • Neurosci Lett. 2013 Aug 26;548:249-54. doi: 10.1016/j.neulet.2013.05.038.
Eun Young Jeong 1,  Sujoeng Kim,  Soonwoong Jung,  Gyeongwha Kim,  Hyeonwi Son,  Dong Hoon Lee,  Gu Seob Roh,  Sang Soo Kang,  Gyeong Jae Cho,  Wan Sung Choi,  Hyun Joon Kim
Affiliations

Affiliation

  • 1 Department of Anatomy and Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University, Jinju, Republic of Korea.
PMID: 23714241 DOI: 10.1016/j.neulet.2013.05.038
Abstract

A previous study using a mouse model of depression showed that chronic immobilization stress (CIS) reduces levels of insulin-like growth factor (IGF)-2, IGF binding protein 2 (IGFBP2), osteoglycin, and fibromodulin in the amygdala. Here, using human neuroblastoma cells, we tested whether these four proteins cooperatively modulate neuronal plasticity. We found that IGF-2 and IGFBP2 synergistically increased neurite outgrowth via enhanced early signaling through the IGF type 1 receptor. Furthermore, we found that osteoglycin, a small leucine-rich proteoglycan, significantly increased IGF-2/IGFPB2-induced neurite outgrowth, but fibromodulin had no effect. We also found that central amygdala neurons of CIS-induced depressive mouse showed a decreased total dendritic length. These findings suggest that CIS-responsive proteins modulate neuronal morphology during chronic stress.

Keywords

IGF-2; IGFBP2; Neurite outgrowth; Osteoglycin; SH-SY5Y.

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