1. Academic Validation
  2. REV-ERBα reduction is associated with clinicopathological features and prognosis in human gastric cancer

REV-ERBα reduction is associated with clinicopathological features and prognosis in human gastric cancer

  • Oncol Lett. 2018 Aug;16(2):1499-1506. doi: 10.3892/ol.2018.8809.
Xiaoshan Wang 1 Nana Wang 2 Xiang Wei 3 Haoyuan Yu 1 Zhengguang Wang 1
Affiliations

Affiliations

  • 1 Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230032, P.R. China.
  • 2 Laboratory of Pathophysiology, School of Life Sciences, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
  • 3 Laboratory of Molecular Biology, Department of Biochemistry, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
Abstract

Gastric Cancer is a serious threat to human health. Nuclear receptor subfamily 1 group D member 1 (REV-ERBα) is a member of the nuclear hormone receptor family that regulates lipid metabolism, inflammatory responses and circadian rhythms. However, the role of REV-ERBα in the pathogenesis of human gastric Cancer is unclear. The present study employed gastric Cancer tissues from 74 patients and determined the association between REV-ERBα expression with clinicopathological variables and prognosis. Furthermore, the association between REV-ERBα and Apoptosis in undifferentiated and moderately differentiated human gastric Cancer cells was determined. It was identified that REV-ERBα expression was decreased in gastric Cancer, which was positively associated with poor differentiation (P=0.009), T stage (P=0.001), Tumor-Node-Metastasis (TMN) stage (P=0.001) and lymph node metastasis (P=0.007). In the survival analysis, the 3- and 5-year survival times of patients were significantly associated with REV-ERBα expression (P=0.009 and P=0.002, respectively). Low REV-ERBα expression was associated with poor prognosis (P<0.05). Concurrently, cleaved Caspase-3 expression was downregulated, whereas expression levels of Bcl-2 and the Bcl-2/Bax ratio were upregulated in gastric Cancer tissues compared with normal tissues. REV-ERBα activator GSK4112 caused Apoptosis in SGC-7901 and BGC-823 cell lines. REV-ERBα levels were decreased in human gastric Cancer, which was associated with poor differentiation, TMN stages and poor prognosis. REV-ERBα is a potential biomarker for tumor development and prognosis, and a potential therapeutic target for gastric Cancer.

Keywords

apoptosis; biomarker; gastric cancer; nuclear receptor subfamily 1 group D member 1; prognosis.

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