2. Academic Validation
  3. miR-129-5p attenuates cell proliferation and epithelial mesenchymal transition via HMGB1 in gastric cancer

miR-129-5p attenuates cell proliferation and epithelial mesenchymal transition via HMGB1 in gastric cancer

  • Pathol Res Pract. 2019 Apr;215(4):676-682. doi: 10.1016/j.prp.2018.12.024.
Shaocheng Wang 1,  Yanyan Chen 1,  Xiongfei Yu 1,  Yimin Lu 1,  Haoao Wang 1,  Fusheng Wu 1,  Lisong Teng 2
Affiliations

Affiliations

  • 1 Department of Surgical Oncology, The First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang, PR China.
  • 2 Department of Surgical Oncology, The First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang, PR China. Electronic address: [email protected].
PMID: 30635217 DOI: 10.1016/j.prp.2018.12.024
Abstract

Background: The miR-129-5p has been reported to be aberrant expression and exert vital roles in tumor progression of various malignancies. However, the effects on EMT in gastric Cancer and its precise molecular mechanism in gastric Cancer remain unclear.

Methods and materials: RT-qPCR was performed to evaluate the expression level of miR-129-5p and HMGB1 in cell lines. Cell proliferation was detected via CCK-8. The epithelial mesenchymal transition (EMT) related proteins and the expression of HMGB1 were detected by western blot analysis. Luciferase assays were used to validate binding seeds between miR-129-5p and HMGB1.

Results: miR-129-5p was downregulated in gastric Cancer cells compared with GES-1. At the same time EMT was promoted in gastric Cancer cells compared to GES-1. Overexpression of miR-129-5p inhibited EMT and proliferation. MiR-129-5p negatively and directly targeted HMGB1. HMGB1 was upregulated in gastric Cancer cells and HMGB1 knocked-down inhibited EMT and cell proliferation.

Conclusion: Taken together, upregulation of miR-129-5p associated with gastric Cancer proliferation and EMT, and serves as a potential diagnostic and therapeutic target via miR-129-5p/HMGB1 pathway in gastric Cancer.

Keywords

Epithelial mesenchymal transition; Gastric cancer; HMGB1; Proliferation; miR-129-5p.

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