2. Academic Validation
  3. Core regulatory RNA molecules identified in articular cartilage stem/progenitor cells during osteoarthritis progression

Core regulatory RNA molecules identified in articular cartilage stem/progenitor cells during osteoarthritis progression

  • Epigenomics. 2019 May;11(6):669-684. doi: 10.2217/epi-2018-0212.
Shuai Zhang 1,  Qier An 1,  Peilin Hu 1,  Xiaomin Wu 2,  Xiaohua Pan 2,  Wenjin Peng 1,  Rikang Wang 1,  Jingyi Gan 1,  Di Chen 3,  Zhen Li 4,  Tianfu Wang 5,  Guangqian Zhou 1
Affiliations

Affiliations

  • 1 Department of Medical Cell Biology & Genetics, Guangdong Key Laboratory of Genomic Stability & Disease Prevention, Shenzhen Key Laboratory of Anti-aging & Regenerative Medicine, & Shenzhen Engineering Laboratory of Regenerative Technologies for Orthopaedic Diseases, Health Sciences Center, Shenzhen University, Shenzhen 518060, PR China.
  • 2 Department of Orthopedic & Traumatology, Shenzhen BaoAn People Hospital Affiliated Southern Medical University, Shenzhen, Guangdong 518101, PR China.
  • 3 Department of Orthopedic Surgery, Rush University Medical Center, Chicago, IL 60612, USA.
  • 4 Shenzhen Alps Cell Sci-Tech Co. Ltd, Longhua District, Shenzhen, PR China.
  • 5 Guangdong Key Laboratory for Biomedical Measurements & Ultrasound Imaging, School of Biomedical Engineering, Health Sciences Center, Shenzhen University, Shenzhen 518060, PR China.
PMID: 30775942 DOI: 10.2217/epi-2018-0212
Abstract

Aim: To assess cartilage-derived stem/progenitor cells (CSPCs) in osteoarthritis (OA) by employing mRNA-miRNA-circRNA-lncRNA network biology approach. Methods: Differentially expressed (DE) RNAs in CSPCs from 2-/4-/8-month-old STR/Ort and CBA mice were identified to construct networks via RNA sequencing. Results: Compared with age-matched CBA mice, 4-/8-month-old STR/Ort mice had cartilage lesions and their CSPCs exhibited lower proliferative and differentiation capacity (decreased CD44 and CD90), and identified 7082 DE RNAs in STR/Ort mice were associated with strain differences or OA progression. OA-related core RNAs were identified via the networks constructed with the predominant DE RNAs, which were involved in the signaling pathways (NF-κB/MAPK/Hippo/Wnt/TGF-β/Cytoskeleton organization). The core RNAs (miR-322-5p/miR-493-5p/miR-378c/CPNE1/Cdh2/PRDM16/CTGF/NCAM1) were validated in CSPCs from OA patients. Conclusion: RNA-based networks identifying core RNAs and signaling pathways contribute to CSPC-dependent OA mechanisms.

Keywords

RNA-sequencing; STR/Ort mice; cartilage; circRNA; lncRNA; mRNA; miRNA; noncoding RNAs; osteoarthritis; proliferation; stem/progenitor cells.

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