1. Academic Validation
  2. Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library

Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library

  • Sci Rep. 2019 Mar 7;9(1):3780. doi: 10.1038/s41598-019-40381-4.
Xi Qin 1 Wenrong Yao 1 Xinchang Shi 1 Lan Liu 1 Fang Huang 2 Youxue Ding 1 Yong Zhou 1 Lei Yu 1 Chuncui Jia 1 Shanhu Li 3 Chunming Rao 4 Junzhi Wang 5
Affiliations

Affiliations

  • 1 Department of Recombinant Products, National Institutes for Food and Drug Control, Beijing, 100050, China.
  • 2 Department of Cell Engineering, Beijing Institute of Biotechnology, Beijing, 100850, China.
  • 3 Department of Cell Engineering, Beijing Institute of Biotechnology, Beijing, 100850, China. [email protected].
  • 4 Department of Recombinant Products, National Institutes for Food and Drug Control, Beijing, 100050, China. [email protected].
  • 5 Department of Recombinant Products, National Institutes for Food and Drug Control, Beijing, 100050, China. [email protected].
Abstract

Bioassay of recombinant protein products is important tests to ensure protein effectiveness. Some recombinant protein products have no cells used in their bioassay but instead use animal models, while Others have no suitable method. Here, we developed a method to obtain responsive cells used in bioassay of proteins. After screening of a CRISPR/Cas9 library, we obtained a responsive cell line that grew faster in the presence of rhEGF (recombinant human epidermal growth factor) than that of control cells. We used this cell line for bioassay of rhEGF. This cell line, compared with the control cells, had a 2 day shorter operation time and had lower interference. The responsive cell line is more suitable for use in bioassay of rhEGF.

Figures
Products