1. Academic Validation
  2. Differentially expressed lncRNA-m433s1 regulates FSH secretion by functioning as a miRNA sponge in male rat anterior pituitary cells†

Differentially expressed lncRNA-m433s1 regulates FSH secretion by functioning as a miRNA sponge in male rat anterior pituitary cells†

  • Biol Reprod. 2019 Aug 1;101(2):416-425. doi: 10.1093/biolre/ioz100.
Dong-Xu Han 1 Xu-Lei Sun 1 Chang-Jiang Wang 1 Ze-Wen Yu 1 Yi Zheng 1 Yi-Jie Huang 1 Wen-Hua Wang 1 Hao Jiang 1 Yan Gao 1 Bao Yuan 1 Jia-Bao Zhang 1
Affiliations

Affiliation

  • 1 Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin, P.R. China.
Abstract

Long noncoding RNAs (lncRNAs) are important regulators that have multiple functions in a variety of biological processes. However, the contributions of lncRNAs to follicle-stimulating hormone (FSH) secretion remain largely unknown. In this study, we first identified a novel lncRNA, lncRNA-m433s1, as an intergenic lncRNA located in the cytoplasm. We next used MS2-RIP assays to demonstrate that lncRNA-m433s1 interacted with miR-433. Furthermore, we detected the levels of lncRNA-m433s1, miR-433, and Fshβ expression, FSH concentrations, and Apoptosis upon overexpression and knockdown of lncRNA-m433s1, revealing that lncRNA-m433s1 upregulated Fshβ expression. Globally, lncRNA-m433s1 reduced the inhibitory effect of miR-433 on Fshβ and further regulated FSH secretion as a competing endogenous RNA (ceRNA) by sponging miR-433. This ceRNA model will provide novel insight into the regulatory mechanisms of lncRNAs associated with rat reproduction.

Keywords

FSH; animal reproduction; lncRNA; miRNA sponge; pituitary.

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