2. Academic Validation
  3. CD10 expression identifies a subset of human perivascular progenitor cells with high proliferation and calcification potentials

CD10 expression identifies a subset of human perivascular progenitor cells with high proliferation and calcification potentials

  • Stem Cells. 2020 Feb;38(2):261-275. doi: 10.1002/stem.3112.
Lijun Ding 1 2 3,  Bianca Vezzani 2 4,  Nusrat Khan 2,  Jing Su 1,  Lu Xu 1,  Guijun Yan 1,  Yong Liu 5,  Ruotian Li 6,  Anushri Gaur 2,  Zhenyu Diao 1,  Yali Hu 1,  Zhongzhou Yang 7,  W Reef Hardy 8,  Aaron W James 8 9,  Haixiang Sun 1 10,  Bruno Péault 2 8
Affiliations

Affiliations

  • 1 Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, People's Republic of China.
  • 2 MRC Center for Regenerative Medicine and Center for Cardiovascular Science, University of Edinburgh, Scotland, UK.
  • 3 Clinical Center for Stem Cell Research, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, People's Republic of China.
  • 4 Department of Morphology, Surgery and Experimental Medicine, Section of General Pathology, University of Ferrara, Ferrara, Italy.
  • 5 Department of Experimental Medicine, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, People's Republic of China.
  • 6 Department of Cardiology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, People's Republic of China.
  • 7 State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing Biomedical Research Institute, Nanjing University, Nanjing, People's Republic of China.
  • 8 Orthopedic Hospital Research Center and Broad Stem Cell Center, David Geffen School of Medicine, University of California, Los Angeles, California.
  • 9 Department of Pathology, Johns Hopkins University, Baltimore, Massachusetts.
  • 10 Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, People's Republic of China.
PMID: 31721342 DOI: 10.1002/stem.3112
Abstract

The tunica adventitia ensheathes arteries and veins and contains presumptive mesenchymal stem cells (MSCs) involved in vascular remodeling. We show here that a subset of human adventitial cells express the CD10/CALLA cell surface metalloprotease. Both CD10+ and CD10- adventitial cells displayed phenotypic features of MSCs when expanded in culture. However, CD10+ adventitial cells exhibited higher proliferation, clonogenic and osteogenic potentials in comparison to their CD10- counterparts. CD10+ adventitial cells increased expression of the cell cycle protein CCND2 via ERK1/2 signaling and osteoblastogenic gene expression via NF-κB signaling. CD10 expression was upregulated in adventitial cells through sonic hedgehog-mediated GLI1 signaling. These results suggest that CD10, which marks rapidly dividing cells in other normal and malignant cell lineages, plays a role in perivascular MSC function and cell fate specification. These findings also point to a role for CD10+ perivascular cells in vascular remodeling and calcification.

Keywords

CD10; GLI1; SHH; adventitia; mesenchymal stem cell; osteogenesis; vascular calcification.

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