1. Academic Validation
  2. Muscle gene expression of CGRP-α, CGRP receptor, nAchR-β, and GDNF in response to different endurance training protocols of Wistar rats

Muscle gene expression of CGRP-α, CGRP receptor, nAchR-β, and GDNF in response to different endurance training protocols of Wistar rats

  • Mol Biol Rep. 2020 Jul;47(7):5305-5314. doi: 10.1007/s11033-020-05610-4.
Ali Gorzi 1 Firooz Jamshidi 2 Ahmad Rahmani 2 Walter Krause Neto 3
Affiliations

Affiliations

  • 1 Department of Sport Sciences, University of Zanjan, P.O. Box: 45371-38791, Zanjan, Iran. [email protected].
  • 2 Department of Sport Sciences, University of Zanjan, P.O. Box: 45371-38791, Zanjan, Iran.
  • 3 Department of Physical Education, Laboratory of Morphoquantitative Studies and Immunohistochemistry, Universidade São Judas Tadeu, São Paulo, Brazil.
Abstract

The neuromuscular junction underwent adaptations to meet the demands of muscles following increased muscle activity. This study aimed to investigate the effects of high-intensity interval training (HIIT), endurance training (END), and mixed interval training (MIX) on the gene expression of the Calcitonin gene-related peptide-α (CGRP-α), CGRP Receptor, nicotinic acetylcholine receptors (nAChR)-β and glial-derived neurotrophic factor (GDNF) among different muscle types. Male Wistar rats were randomly divided into four groups: Control (n = 8), END (n = 8), HIIT (n = 8), and MIX (n = 8). The Animals run each training protocol for 8 weeks (five sessions/week). Forty-eight hours after the last training session, the muscles gastrocnemius and soleus were excised under the sterilized situation. After collection, the material was prepared for RNA extraction, Reverse Transcriptase reaction, and qPCR assay. The HIIT training up-regulated the CGRP-α (p < 0.01), CGRP-Rec (p < 0.01), and GDNF (p < 0.01) in soleus as well as the nAchR-β (p < 0.01) and GDNF (p < 0.01) in gastrocnemius muscles. END training down-regulated the gene expression of CGRP-α (p < 0.01), and nAchR-β (p < 0.01) in gastrocnemius but up-regulated nAchR-β (p = 0.037) in soleus and GDNF (p < 0.01) in gastrocnemius muscles. MIX training did not show any significant up or down-regulation. The endurance performance of HIIT and MIX groups was higher than the END group (p < 0.01). All studied genes up-regulated by HIIT training in a muscle type-specific manner. It seems that the improvement of some synaptic indices induced by HIIT resulted in the improvement of endurance performance.

Keywords

Calcitonin gene-related peptide; Neuromuscular facilitation; Strenuous training; Synaptic transmission.

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