1. Academic Validation
  2. Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines

Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines

  • Evid Based Complement Alternat Med. 2020 Oct 14;2020:4979850. doi: 10.1155/2020/4979850.
Qianli Kang 1 Yanyan Wang 1 Qinghua Cui 1 2 3 Lili Gong 1 2 4 Yong Yang 2 4 Haiqiang Jiang 1 4 Lijun Rong 5 Rong Rong 1 2 Ruikun Du 3 4
Affiliations

Affiliations

  • 1 College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China.
  • 2 Shandong Provincial Collaborative Innovation Center for Antiviral Traditional Chinese Medicine, Jinan 250355, Shandong, China.
  • 3 Qingdao Academy of Chinese Medicinal Sciences, Shandong University of Traditional Chinese Medicine, Qingdao 266122, Shandong, China.
  • 4 Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China.
  • 5 Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago 60612, IL, USA.
Abstract

Traditional Chinese medicines (TCMs) have proven to possess advantages in counteracting virus infections according to clinical practices. It's therefore of great value to discover novel antivirals from TCMs. In this paper, One hundred medicinal Plants which have been included in TCM prescriptions for Antiviral treatment were selected and prefractionated into 5 fractions each by sequentially using cyclohexane, dichloromethane, ethyl acetate, n-butanol, and water. 500 TCM-simplified extracts were then subjected to a phenotypic screening using a recombinant IAV expressing Gaussia luciferase. Ten TCM fractions were identified to possess Antiviral activities against Influenza Virus. The IC50's of the hit fractions range from 1.08 to 6.45 μg/mL, while the SIs, from 7.52 to 98.40. Furthermore, all the ten hit fractions inhibited the propagation of progeny Influenza Virus significantly at 20 μg/mL. The hit TCM fractions deserve further isolation for responsible constituents leading towards anti-influenza drugs. Moreover, a library consisting of 500 simplified TCM extracts was established, facilitating Antiviral screening in quick response to emerging and re-emerging viruses such as Ebola virus and current SARS-CoV-2 pandemic.

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