1. Academic Validation
  2. Investigating the effect of lncRNA HOTAIR on apoptosis induced by myocardial ischemia-reperfusion injury

Investigating the effect of lncRNA HOTAIR on apoptosis induced by myocardial ischemia-reperfusion injury

  • Mol Med Rep. 2021 Mar;23(3):169. doi: 10.3892/mmr.2020.11808.
Jijing Fang 1 Weihong Zheng 2 Pengfei Hu 3 Jiale Wu 4
Affiliations

Affiliations

  • 1 Department of Rehabilitation Medicine, The First People's Hospital of Tonglu, Tonglu County, Hangzhou, Zhejiang 311500, P.R. China.
  • 2 School of Life Science, Huzhou University, Huzhou, Zhejiang 313000, P.R. China.
  • 3 Department of Cardiology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310005, P.R. China.
  • 4 Department of Geratology, Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang 310007, P.R. China.
Abstract

The present study aimed to investigate the effect of the long non‑coding ribonucleic acid (lncRNA) HOX transcript antisense intergenic RNA (HOTAIR) on Apoptosis induced by ischemia‑reperfusion injury. Differential lncRNAs in myocardial ischemia rats were screened by a lncRNA microarray and the expression levels of lncRNA HOTAIR and MicroRNA (miR)‑130a‑3p were analyzed using reverse transcription‑quantitative polymerase chain reaction in hypoxia‑induced cardiomyocytes. The mechanism of lncRNA HOTAIR in cardiotoxicity was investigated using Cell Transfection, lncRNA knockdown, Cell Counting Kit‑8, flow cytometry, western blotting, dual luciferase reporter assays and RNA immunoprecipitation. The expression level of lncRNA HOTAIR was significantly downregulated in the ischemic myocardium of rats. Overexpression of HOTAIR in H9c2 (rat cardiomyocyte line) cells could inhibit the Apoptosis induced by H2O2. A direct interaction was found between HOTAIR and miR‑130a‑3p, and mouse double minute 4 (MDM4) was also found to be a potential target of miR‑130a‑3p. The overexpression of MDM4 in H9c2 cells transfected with miR‑130a‑3p mimics increased Apoptosis, and miR‑130a‑3p targeted inhibition of MDM4 promoted H2O2‑induced Apoptosis of H9c2 cells. Overall, HOTAIR was found to inhibit the Apoptosis of H9c2 cells induced by H2O2 through the miR‑130a‑3p/MDM4 axis.

Keywords

long non‑coding RNA; HOX transcript antisense intergenic RNA; microRNA‑130a‑3p; mouse double minute 4; myocardial ischemia‑reperfusion injury.

Figures
Products