1. Academic Validation
  2. Respiratory exposure to PM2.5 soluble extract induced chronic lung injury by disturbing the phagocytosis function of macrophage

Respiratory exposure to PM2.5 soluble extract induced chronic lung injury by disturbing the phagocytosis function of macrophage

  • Environ Sci Pollut Res Int. 2022 Feb;29(10):13983-13997. doi: 10.1007/s11356-021-16797-9.
Chen Zhao  # 1 Wenyuan Pu  # 1 Mengyuan Niu 1 Junaid Wazir 1 Shiyu Song 1 Lulu Wei 1 Li Li 1 Zhonglan Su 2 Hongwei Wang 3
Affiliations

Affiliations

  • 1 State Key Laboratory of Analytical Chemistry for Life Science & Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, Nanjing, 210093, People's Republic of China.
  • 2 Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, People's Republic of China. [email protected].
  • 3 State Key Laboratory of Analytical Chemistry for Life Science & Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, Nanjing, 210093, People's Republic of China. [email protected].
  • # Contributed equally.
Abstract

Exposure to airborne urban particles is a contributing factor for the development of multiple types of respiratory diseases; its pathological role as a cause of lung injury is still unclear. In this study, PM2.5 soluble extract was collected, and its toxicological effect on lung pathological changes was examined. To assess its pathological mechanism, Human Monocyte-Like Cell Line, THP-1, and mouse macrophage, RAW264.7, were used to determine the effects of PM2.5 soluble extract on cell toxicity, phagocytosis, and transcriptome. We found that PM2.5 soluble extract exposure activated NF-κB and MAPK signaling pathways, then induces the production of pro-inflammatory cytokines. RNA-seq results showed that the transcription profiles, including 1213 genes, have been changed in responses to PM2.5 exposure. Additionally, PM2.5 led to phagocytic dysfunction, which may exacerbate the cause of lung injury. Exposure to PM2.5 soluble extract triggers the death of respiratory macrophages, impairs its phagocytosis capacity, thus delaying the inflammatory cell clearance in the lung, which results in chronic lung injury.

Keywords

Inflammation; Lung injury; PM2.5; Phagocytosis.

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