2. Academic Validation
  3. Trehalose inhibits proliferation while activates apoptosis and autophagy in rat airway smooth muscle cells

Trehalose inhibits proliferation while activates apoptosis and autophagy in rat airway smooth muscle cells

  • Acta Histochem. 2021 Dec;123(8):151810. doi: 10.1016/j.acthis.2021.151810.
Bo Xiao 1,  Haiming Huang 2,  Liangxian Li 3,  Lixia Hou 2,  Dong Yao 4,  Biwen Mo 5
Affiliations

Affiliations

  • 1 Laboratory of Respiratory Disease, Affiliated Hospital of Guilin Medical University, China. Electronic address: [email protected].
  • 2 Laboratory of Respiratory Disease, Affiliated Hospital of Guilin Medical University, China.
  • 3 Guangxi Key Laboratory of Brain and Cognitive Neuroscience, Guilin Medical University, China.
  • 4 Laboratory of Respiratory Disease, Affiliated Hospital of Guilin Medical University, China. Electronic address: [email protected].
  • 5 Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Guilin Medical University, China. Electronic address: [email protected].
PMID: 34749031 DOI: 10.1016/j.acthis.2021.151810
Abstract

Trehalose is a disaccharide with multiple important biological activities. In many cell types, Trehalose regulates the physiological behaviors of proliferation, Apoptosis and Autophagy. But the effects of trehalose on ASMCs have never been reported. Here, we showed that trehalose activated Autophagy of ASMCs at low dose, inhibited proliferation and induced Apoptosis of ASMCs at high dose. Further study, we found the cell cycle was arrested in S and G2\M phases, the expression of CyclinA1 and CyclinB1 decreased. Then, we investigated the ratio of Bcl-2/Bax was drastically reduced. Next, we detected an important transcription factor TFEB, which is closely related to Autophagy. We found TFEB was highly activated with trehalose treatment. And many downstream autophagy-related genes of TFEB were also up-regulated. In summary, trehalose plays an important role on the regulation of proliferation, Apoptosis and Autophagy of ASMCs.

Keywords

ASMCs; Bcl-2 - Bax signal; Cell cycle; TFEB; Trehalose.

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