Peste des petits ruminants virus induces ERS-mediated autophagy to promote virus replication

Peste des petits ruminants virus (PPRV) has long been a significant threat to small ruminant productivity worldwide. Virus infection-induced endoplasmic reticulum (ER) stress (ERS) and the subsequently activated unfolded protein response (UPR) play significant roles in viral replication and pathogenesis. However, the relationship between ERS and PPRV Infection is unknown. In this study, we demonstrated that ERS was induced during PPRV Infection in caprine endometrial epithelial cells (EECs). Importantly, we demonstrated that the induction of Autophagy by PPRV was mediated by ERS. Furthermore, we found that the PERK/eIF2α pathway but not the ATF6 or IRE1 pathway was activated and that the activated PERK/eIF2α pathway participated in regulating ERS-mediated Autophagy. Moreover, virus replication was required for PPRV infection-induced ERS-mediated Autophagy and PERK pathway activation. Additionally, we revealed that either the viral nucleocapsid (N) or nonstructural protein C was sufficient to elicit ERS and activate the PERK/eIF2α pathway, which further increased Autophagy. Taken together, these results suggest that PPRV N and C protein-induced Autophagy enhances viral replication through the induction of ERS and that the PERK pathway may be involved in the activation of ERS-mediated Autophagy during PPRV Infection.