2. Academic Validation
  3. Ameliorative effect of the probiotic peptide against benzo(α)pyrene-induced inflammatory damages in enterocytes

Ameliorative effect of the probiotic peptide against benzo(α)pyrene-induced inflammatory damages in enterocytes

  • Int Immunopharmacol. 2022 Sep 21;112:109255. doi: 10.1016/j.intimp.2022.109255.
Min Luo 1,  Dan Luo 1,  Jie Liu 2,  Huailing Wang 2,  Xiaoyu Liu 2,  Min Yang 3,  Fangfang Tian 3,  Suofu Qin 3,  Yuying Li 4
Affiliations

Affiliations

  • 1 Laboratory of inflammation and allergy, Department of respiratory and critical care medicine, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
  • 2 State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen 518060, China.
  • 3 Kexing Biopharm Co., Ltd, Shenzhen 518057, China.
  • 4 Laboratory of inflammation and allergy, Department of respiratory and critical care medicine, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China. Electronic address: [email protected].
PMID: 36152539 DOI: 10.1016/j.intimp.2022.109255
Abstract

Probiotics are living bacteria that provide health benefits to the host when consumed in sufficient quantities. However, the protective effect of the bioactive Peptides isolated from the probiotics against benzo(α)pyrene (BaP) induced gastrointestinal injury has never been investigated. The current work used a bio-assay guided technique to identify-four new cyclic Peptides in BaP-induced Caco-2 Cell Culture and mouse colitis model. Lactobacillus rhamnosus cycle (Thr-His-Ala-Trp) peptide-1 (LRCP-1) effectively inhibited BaP-induced epithelial cytokine over-release and intracellular ROS over-production. Simultaneously, LRCP-1 attenuated BaP-induced NAD (P)H: oxidases (NOXs), Matrix Metalloproteinases (MMPs) over-expression, respectively. Furthermore, increased NAD (P)H: quinone oxidoreductase 1 (NQO1)/heme oxygenase-1 (HO-1)/nuclear factor E2-related factor 2 (Nrf2) expression and Aryl Hydrocarbon Receptor (AhR) pathway activation induced by the BaP-exposure were also inhibited after the LRCP-1 treatment. Notably, LRCP-1 is a promising agent protecting gastrointestinal epithelial cells from BaP-induced inflammatory and oxidative damages.

Keywords

AhR; Benzo(α)pyrene; Cyclic peptide; Lactobacillus Rhamnosus.

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