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  2. Ectoderm mesenchymal stem cells promote osteogenic differentiation of MC3T3-E1 cells by targeting sonic hedgehog signaling pathway

Ectoderm mesenchymal stem cells promote osteogenic differentiation of MC3T3-E1 cells by targeting sonic hedgehog signaling pathway

  • Mol Biol Rep. 2022 Dec 1. doi: 10.1007/s11033-022-08022-8.
Lu Bian # 1 2 YiQing Wu # 1 2 Jiawei Wu # 1 2 Peng Zhao 3 4 Xijiang Zhao 5 6
Affiliations

Affiliations

  • 1 Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu Province, China.
  • 2 Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu Province, China.
  • 3 Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu Province, China. [email protected].
  • 4 Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu Province, China. [email protected].
  • 5 Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu Province, China. [email protected].
  • 6 Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu Province, China. [email protected].
  • # Contributed equally.
Abstract

Background: Despite their high repair capability, bone defects still present a major challenge in orthopedic tissue engineering. Osteoblast differentiation is central to the treatment of bone defects. METHODS AND RESULTS: We used nasal mucosal-derived ectoderm mesenchymal stem cells (EMSCs) to promote osteogenic differentiation by co-culturing MC3T3-E1 cells. Our results showed that MC3T3-E1/EMSCs co-culture upregulated bone-related proteins and transglutaminase 2 (TG2) and increased Alkaline Phosphatase (ALP) activity and bone nodule formation relative to controls. Furthermore, our results showed that EMSC-derived sonic Hedgehog (Shh) accounted for the enhanced MC3T3-E1 differentiation because inhibiting Shh signaling substantially reduced osteogenic differentiation.

Conclusion: Altogether, these results suggest that EMSCs differentiated into osteoblast cells and supported MC3T3-E1 differentiation. Thus, EMSCs may be a promising cell source for treating bone-related diseases.

Keywords

EMSCs; Osteogenic differentiation; Shh signaling; TG2.

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