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MYLS22 is a selective optic atrophy 1OPA1 inhibitor. MYLS22 reduces tumor vascularization and associated lymphatic angiogenesis by inhibiting OPA1, thereby limiting tumor growth and metastasis and effectively normalizing tumor vascular morphology. MYLS22 has anticancer activity.

For research use only. We do not sell to patients.

MYLS22 Chemical Structure

MYLS22 Chemical Structure

CAS No. : 306959-01-3

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 79 In-stock
Solution
10 mM * 1 mL in DMSO USD 79 In-stock
Solid
1 mg USD 34 In-stock
5 mg USD 72 In-stock
10 mg USD 121 In-stock
50 mg USD 308 In-stock
100 mg USD 490 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 8 publication(s) in Google Scholar

Top Publications Citing Use of Products

    MYLS22 purchased from MedChemExpress. Usage Cited in: Leukemia. 2023 Feb 4.  [Abstract]

    MYLS22 (10-30 μM) significantly reduces mitochondrial length and decreases ROS content in PDX AML cells. Quantification of mitochondrial length using MTDR/DAPI staining and confocal imaging.

    MYLS22 purchased from MedChemExpress. Usage Cited in: Leukemia. 2023 Feb 4.  [Abstract]

    MYLS22 (0-100 μM; 48 h) decreases the viability of MOLM-14 cells by 40% (IC50 = 2.95 μM).
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    MYLS22 is a selective optic atrophy 1OPA1 inhibitor. MYLS22 reduces tumor vascularization and associated lymphatic angiogenesis by inhibiting OPA1, thereby limiting tumor growth and metastasis and effectively normalizing tumor vascular morphology. MYLS22 has anticancer activity[1][2][3].

    IC50 & Target

    OPA1[1]

    In Vitro

    MYLS22 (10-30 μM, 7-10 days) inhibits the clonal growth of acute myeloid leukemia (AML) cells with an IC50 of 12.5 μM[1].
    MYLS22 (10-30 μM, 7-10 days) significantly inhibits the mitochondrial fusion of AML cells, thereby reducing the ROS content and inhibiting the cell cycle in transition stage of G0/G1 transition stage[1].
    MYLS22 (10 nM, 30 min) aggravates necroptosis of AECs under the LPS challenge in vitro in MLE12 cells[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Western Blot Analysis[4]

    Cell Line: MLE12 cells
    Concentration: 10 nM
    Incubation Time: 30 min
    Result: Increased the phosphorylation levels of RIPK3 and MLKL protein.
    In Vivo

    MYLS22 (30 mg/kg, Intraperitoneal injection, Once daily for 7 days) significantly reduces the total tumor load of leukemia mice [1].
    MYLS22 (10 mg/kg, Paratumoral injection, Once every two days for six days) inhibits tumor growth in mice with subcutaneous B16F10 melanoma tumors by targeting Opa1[3].
    MYLS22 (12.5 mg/kg, i.p., once time) aggravates the pathological injury and inflammatory response in the lungs of LPS‐induced ALI mice, which also can aggravate necroptosis of AECs[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: LPS‐induced ALI mice[4]
    Dosage: 12.5 mg/kg
    Administration: i.p., 2 days
    Result: Increased lung inflammatory score, Tnf‐α and pro‐Il‐1β mRNA expression, and total IL‐1β or IL‐1β p17 protein expression.
    Increased the levels of RIPK3, MLKL protein, and their phosphorylation.
    Decreased the levels of SPC protein.
    Molecular Weight

    443.52

    Formula

    C24H21N5O2S

    CAS No.
    Appearance

    Solid

    Color

    Off-white to yellow

    SMILES

    O=C(C(S1)=CC2=C1N(C3=CC=CC=C3)N=C2C)NC4=C(C)N(C)N(C5=CC=CC=C5)C4=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (56.37 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2547 mL 11.2734 mL 22.5469 mL
    5 mM 0.4509 mL 2.2547 mL 4.5094 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1.25 mg/mL (2.82 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 1.25 mg/mL (2.82 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.47%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.2547 mL 11.2734 mL 22.5469 mL 56.3672 mL
    5 mM 0.4509 mL 2.2547 mL 4.5094 mL 11.2734 mL
    10 mM 0.2255 mL 1.1273 mL 2.2547 mL 5.6367 mL
    15 mM 0.1503 mL 0.7516 mL 1.5031 mL 3.7578 mL
    20 mM 0.1127 mL 0.5637 mL 1.1273 mL 2.8184 mL
    25 mM 0.0902 mL 0.4509 mL 0.9019 mL 2.2547 mL
    30 mM 0.0752 mL 0.3758 mL 0.7516 mL 1.8789 mL
    40 mM 0.0564 mL 0.2818 mL 0.5637 mL 1.4092 mL
    50 mM 0.0451 mL 0.2255 mL 0.4509 mL 1.1273 mL
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    MYLS22 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
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    Cat. No.:
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