1. Cell Cycle/DNA Damage
    Epigenetics
    Autophagy
    Anti-infection
    Apoptosis
  2. HDAC
    Autophagy
    HIV
    Apoptosis
  3. Panobinostat

Panobinostat (Synonyms: LBH589; NVP-LBH589)

Cat. No.: HY-10224 Purity: 99.20%
Handling Instructions

Panobinostat (LBH589; NVP-LBH589) est un inhibiteur HDAC puissant et oralement non sélectif, et possède des activités antinéoplasiques. Panobinostat induit la production du virus VIH-1 même à une faible concentration de 8 à 31 nM, stimule l'expression du VIH-1 dans les cellules infectées de manière latente. Panobinostat induit l'apoptose et l'autophagie des cellules. Panobinostat peut être utilisé pour l'étude du myélome multiple réfractaire ou en rechute.

Panobinostat (LBH589; NVP-LBH589) ist ein potenter und oral wirksamer, nicht-selektiver HDAC-Inhibitor mit antineoplastischen Aktivitäten. Panobinostat induziert die Produktion des HIV-1 virus selbst im niedrigen Konzentrationsbereich von 8-31 nM und stimuliert die HIV-1-Expression in latent infizierten Zellen. Panobinostat induziert apoptosis und autophagy. Panobinostat kann für die Untersuchung von refraktären oder rezidivierten Multiplen Myelomen verwendet werden.

Panobinostat (LBH589; NVP-LBH589) is a potent and orally active non-selective HDAC inhibitor, and has antineoplastic activities. Panobinostat induces HIV-1 virus production even at low concentration range 8-31 nM, stimulates HIV-1 expression in latently infected cells. Panobinostat induces cell apoptosis and autophagy. Panobinostat can be used for the study of refractory or relapsed multiple myeloma.

For research use only. We do not sell to patients.

Panobinostat Chemical Structure

Panobinostat Chemical Structure

CAS No. : 404950-80-7

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1  mL in DMSO USD 73 In-stock
Estimated Time of Arrival: December 31
10 mg USD 66 In-stock
Estimated Time of Arrival: December 31
50 mg USD 132 In-stock
Estimated Time of Arrival: December 31
100 mg USD 180 In-stock
Estimated Time of Arrival: December 31
200 mg USD 228 In-stock
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500 mg USD 468 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 14 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Panobinostat purchased from MCE. Usage Cited in: China Biotechnology. 2016, 36(6): 9-17.

    The HDAC expression of PC3 cell with different concentration of Panobinostat. (a) HDACs mRNA expression is analyzed by real time PCR (b) HDACs protein expression is analyzed by Western blot.

    Panobinostat purchased from MCE. Usage Cited in: PLoS Pathog. 2018 Sep 13;14(9):e1007267. 

    Immunoblot analysis for vIL-6, ORF45, or actin is performed on lysates from BCBL-1 cells treated with NaB+TPA, Romidepsin or Panobinostat.
    • Biological Activity

    • Protocol

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    • References

    • Customer Review

    Description

    Panobinostat (LBH589; NVP-LBH589) is a potent and orally active non-selective HDAC inhibitor, and has antineoplastic activities[1][2]. Panobinostat induces HIV-1 virus production even at low concentration range 8-31 nM, stimulates HIV-1 expression in latently infected cells[4]. Panobinostat induces cell apoptosis and autophagy. Panobinostat can be used for the study of refractory or relapsed multiple myeloma[3].

    IC50 & Target

    HDAC

     

    In Vitro

    Panobinosta (LBH589) induces apoptosis of both MOLT-4 and Reh cells in a time- and dose-dependent manner. Panobinosta treatment results in histone (H3K9 and H4K8) hyperacetylation and regulation of cell-cycle control genes in Reh cells[1]. Panobinostat exhibites potent antiproliferative activity in human NSCLC cell lines with the IC50 ranging from 5 to 100 nM[2].

    In Vivo

    Panobinosta (10, 20 mg/kg, i.p.) significantly slows tumor growth derived from Meso and NSCLC cells in vivo models. Panobinosta markedly increases acetylation of histone H3 and H4 of H69 human SCLC cells harvest from SCID mice[2]. Panobinostat (5, 10 and 20 mg/kg i.p.) demonstrates a clear benefit of decreased tumor burden, significantly improves TTE and reduces bone density loss in a disseminated multiple myeloma mouse model[3].

    Clinical Trial
    Molecular Weight

    349.43

    Formula

    C₂₁H₂₃N₃O₂

    CAS No.

    404950-80-7

    SMILES

    O=C(/C=C/C1=CC=C(CNCCC2=C(NC3=C2C=CC=C3)C)C=C1)NO

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 57 mg/mL (163.12 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8618 mL 14.3090 mL 28.6180 mL
    5 mM 0.5724 mL 2.8618 mL 5.7236 mL
    10 mM 0.2862 mL 1.4309 mL 2.8618 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (7.15 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (7.15 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (7.15 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Cell Assay
    [1]

    Cells are washed with ice-cold PBS containing 0.1 mM sodium orthovanadate, and total proteins are isolated using RIPA lysis buffer, which includes protease inhibitors (leupeptin, antipain, and aprotinin), 0.5 mM PMSF, and 0.2 mM sodium orthovanadate. Protein amounts are quantified using the Bio-Rad protein assay. Equal amounts of proteins are loaded onto an sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel, transferred onto nitrocellulose membrane, and probed with the antibody of interest: mouse monoclonal c-Myc and mouse monoclonal p21 antibodies; rabbit polyclonal phospho-Histone H2A.X, rabbit polyclonal acetyl-Histone H3 (Lys9), and rabbit polyclonal acetyl-Histone H4 (Lys8) antibodies; mouse monoclonal p27/KIP1 antibody; mouse monoclonal anti-β-actin; and mouse monoclonal anti-GADD45G. Membranes are then washed, reprobed with appropriate horseradish peroxidase-conjugated secondary antibodies, and developed with SuperSignal chemiluminescent substrate.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [1]

    AE17 and TC-1 cancer cells (1×106 cells) are injected into the flanks of adult female C57Bl/6 mice and severe combined immunodeficiency (SCID) mice. M30 (10×106 cells), A549 (5×106 cells), H69 (2.5×106 cells), BK-T (6.5×106), H526 (10×106), and RG1 (10×106) cells are also injected, but in the presence of matrigel, into the flanks of SCID mice. When tumors reach 100 to 500 mm3, panobinostat is administered via i.p. injections (10-20 mg/kg) on a daily schedule (5-days-on, 2-days-off regimen) for the entire duration of the experiment. Control micereceive i.p. injections with dextrose 5% in water. Every tumor is measured with a caliper at least twice weekly. For evaluation of the effects of combination therapy on SCLC-derived tumors, SCID mice with H69 tumors are administered panobinostat. Three days after the initiation of panobinostat, and again 1 wk later, etoposide (40 mg/kg) is administered i.p.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 99.20%

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    Keywords:

    PanobinostatLBH589NVP-LBH589LBH 589LBH-589HDACAutophagyHIVApoptosisHistone deacetylasesHuman immunodeficiency virusInhibitorinhibitorinhibit

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    Product name:
    Panobinostat
    Cat. No.:
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