1. Metabolic Enzyme/Protease
  2. Cytochrome P450
  3. Pregnenolone 16α-carbonitrile

Pregnenolone 16α-carbonitrile 

Cat. No.: HY-131723
Handling Instructions

Pregnenolone 16α-carbonitrile is an orally active prototypical and effective rodent-PXR activator. Pregnenolone 16α-carbonitrile, a synthetic steroid, induces cytochrome P4503A expression. Pregnenolone 16α-carbonitrile exhibits increased resistance to subsequent stressful insults.

For research use only. We do not sell to patients.

Pregnenolone 16α-carbonitrile Chemical Structure

Pregnenolone 16α-carbonitrile Chemical Structure

CAS No. : 1434-54-4

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Description

Pregnenolone 16α-carbonitrile is an orally active prototypical and effective rodent-PXR activator. Pregnenolone 16α-carbonitrile, a synthetic steroid, induces cytochrome P4503A expression. Pregnenolone 16α-carbonitrile exhibits increased resistance to subsequent stressful insults[1][2][3].

In Vitro

Pregnenolone 16α-carbonitrile (20 μM; 9 days) directly inhibits rat hepatic stellate cell (HSCs) transdifferentiation to a profibrogenic phenotype, although the cells did not express the PXR (in contrast with hepatocytes)[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Pregnenolone 16α-carbonitrile (40 mg/kg/day; i.p.; for two days) induces the expression of Cyp3a11 and Cyp2b10 at the mRNA, protein, and enzymatic levels in WT mice[1].
Pregnenolone 16α-carbonitrile (100 mg/kg; ip; single dose) induces the expression of CYP3A mRNA in adult female Sprague-Dawley rats weighing 150-200 g[2].
Pregnenolone 16α-carbonitrile (35 mg/kg; gavage; once daily for three days) increase in Pgp expression in male Sprague-Dawley rats, aged approximately 100 days and weighing 250-400 g[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: WT and Pxr-/- mice[1]
Dosage: 40 mg/kg
Administration: IP; per day for two days
Result: Induced the expression of Cyp3a11 and Cyp2b10 at the mRNA, protein, and enzymatic levels in WT mice.
Had little effect on the expression of Cyp3a11 in Pxr-/- mice
Molecular Weight

341.49

Formula

C₂₂H₃₁NO₂

CAS No.
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Product Name:
Pregnenolone 16α-carbonitrile
Cat. No.:
HY-131723
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