1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. TNF Superfamily
  4. TNF Superfamily Receptors

TNF Superfamily Receptors

TNF receptor superfamily (TNFRSF) members are transmembrane proteins cysteine-rich motifs in their extracellular domains that bind to their cognate ligands. About 30 members of TNFRSF members have been identified. TNFRs are primarily type I transmembrane proteins that contain an extracellular TNF-binding region and a cytoplasmic tail. But some lack a signal peptide sequence, B-cell maturation antigen (BCMA), transmembrane activator and cyclophilin ligano interactor (TACI), BAFF receptor (BAFFR) and X-linked EDA receptor (XEDAR) belong to type III transmembrane proteins. TNFRs fall into three groups: (1) death domain (DD) containing receptors (fatty acid synthase, TNFR1, and DR3), which activate the caspase cascade via the DD-initiating extrinsic apoptotic pathway; (2) decoy receptors, which lack a cytoplasmic tail and therefore cannot transmit the signal, making these receptors ligand sequesters; and (3) TNFR-associated factor (TRAF) receptors, which lack the DD-recruiting TRAF proteins, such as TNFR2. (Targeting TNF Receptor )

Cat. No. Product Name / Synonyms Species Source
  • HY-P70536
    TNF RII/TNFRSF1B Protein, Human (183a.a, HEK293, mFc)

    TNF-RII; TNFRII; TNF RII; Tumor necrosis factor receptor superfamily member 1B; TNFRSF1B; Tumor necrosis factor receptor 2; TNF-R2; Tumor necrosis factor receptor type II; p75; p80 TNF-alpha receptor; CD120b

    Human HEK 293
  • HY-P72423
    TRAIL R2/TNFRSF10B Protein, Human (Biotinylated, HEK293, Avi-His)

    TNF-Related Apoptosis-Inducing Ligand Receptor 2; TRAIL Receptor 2; TRAIL-R2; CD262; TNFRSF10B; DR5; KILLER; TRICK2; ZTNFR9

    Human HEK 293
  • HY-P70821
    TRAIL Protein, Mouse (HEK 293, Fc)

    Tumor necrosis factor receptor superfamily member 10B/Death receptor 5/MK/CD262/Tnfrsf10b/Dr5/Killer

    Mouse HEK293
  • HY-P70801
    TNFRSF1A Protein, Human (His)

    Tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor 1; TNF-R1; Tumor necrosis factor receptor type I; TNF-RI; TNFR-I; TNFAR; TNFR1

    Human E. coli
  • HY-P71017
    TNFRSF11B/OPG Protein, Mouse (HEK 293, His)

    Tumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; Tnfrsf11b; Ocif; Opg

    Mouse HEK293
  • HY-P70345
    TNFRSF3 Protein, Human (HEK 293, His)

    rHuTumor necrosis factor receptor superfamily member 3/TNFRSF3, His; Tumor Necrosis Factor Receptor Superfamily Member 3; Lymphotoxin-Beta Receptor; Tumor Necrosis Factor C Receptor; Tumor Necrosis Factor Receptor 2-Related Protein; Tumor Necrosis Factor Receptor Type III; TNF-RIII; TNFR-III; LTBR; D12S370; TNFCR; TNFR3; TNFRSF3

    Human HEK293
  • HY-P70363
    TNFRSF3/LTBR Protein, Mouse (HEK 293, Fc)

    rMuTumor necrosis factor receptor superfamily member 3/LTBR, Fc; Tumor necrosis factor receptor superfamily member 3; Lymphotoxin-beta receptor; Ltbr; Tnfcr; Tnfrsf3

    Mouse HEK293
  • HY-P7168
    Fas/CD95 Protein, Human (HEK293)

    rHuFASR/TNFRSF6; Tumor necrosis factor receptor superfamily member 6; Fas receptor; CD95; APT1; FAS1; Apo-1

    Human HEK293
    Fas/CD95 Protein, Human (HEK293) belongs to the subgroup of the tumor necrosis factor receptor (TNF-R) family that can trigger apoptosis.
  • HY-P7169
    Fas/CD95 Protein, Human

    rHuFASR/TNFRSF6; Tumor necrosis factor receptor superfamily member 6; Fas receptor; CD95; APT1; FAS1; Apo-1

    Human E. coli
    Fas/CD95 Protein, Human belongs to the subgroup of the tumor necrosis factor receptor (TNF-R) family that can trigger apoptosis.
  • HY-P72484
    RANK/TNFRSF11A Protein, Human (HEK 293, His)

    Tumor necrosis factor receptor superfamily member 11A; ODFR; CD265; Tnfrsf11a; Rank

    Human HEK293
  • HY-P70023
    CD30/TNFRSF8 Protein, Human (361a.a, HEK293, His)

    rHuTumor necrosis factor receptor superfamily member 8/CD30, His; Tumor necrosis factor receptor superfamily member 8; CD30L receptor; Ki-1 antigen; Lymphocyte activation antigen CD30; CD30; TNFRSF8

    Human HEK 293
  • HY-P70075
    CD30/TNFRSF8 Protein, Mouse (263a.a, HEK293, His)

    rMuTumor necrosis factor receptor superfamily member 8/TNFRSF8, His; Tumor necrosis factor receptor superfamily member 8; CD30L receptor; Lymphocyte activation antigen CD30; CD30; Tnfrsf8

    Mouse HEK 293
  • HY-P70115
    EDAR Protein, Mouse (HEK293, Fc)

    rMuTumor necrosis factor receptor superfamily member EDAR/EDAR, Fc; Tumor necrosis factor receptor superfamily member EDAR; Anhidrotic ectodysplasin receptor 1; Downless; Ectodermal dysplasia receptor; Ectodysplasin-A receptor

    Mouse HEK 293
  • HY-P70329
    Fas/CD95 Protein, Human (HEK 293, His)

    rHuTumor necrosis factor receptor superfamily member 6/TNFRSF6, His; Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; APT1; FAS1; TNFRSF6 and FAS

    Human HEK293
  • HY-P70330
    Fas/CD95 Protein, Mouse (HEK 293, His)

    rMuTumor necrosis factor receptor superfamily member 6//TNFRSF6, His; Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD95; Fas; TNFRSF6

    Mouse HEK293
  • HY-P70516
    TNFRII Protein, Mouse (HEK 293, His)

    Tumor necrosis factor receptor superfamily member 1b; Tnfrsf1b; TNF-RII; TNFRII; TNF RII

    Mouse HEK293
  • HY-P7053
    TNFRSF11B/OPG Protein, Human (CHO, Fc)

    rHuOPG; Tumor necrosis factor receptor superfamily member 11B; TNFRSF11B; OCIF

    Human CHO
    TNFRSF11B/OPG Protein, Human is a decoy receptor for the Receptor Activator of Nuclear Factor-kB ligand (RANKL). It is a member of the TNF receptor superfamily.
  • HY-P70678
    4-1BBR/TNFRSF9 Protein, Human (163a.a, HEK 293, His)

    CD137; ILA; TNFRSF9; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA

    Human HEK293
    4-1BBR/TNFRSF9 Protein, Human (HEK 293, His) is a recombinant Human 4-1BBR/TNFRSF9 Protein expressed in HEK 293 cells with a His tag at the N-terminus. Recombinant Human 4-1BBRTNFRSF9, an inducible T cell molecule belonging to the TNF receptor superfamily, could promote the expansion of antigen-specific T cells and prevent activation-induced death of CD8+ T cells.
  • HY-P70681
    4-1BBR/TNFRSF9 Protein, Human (163a.a, HEK 293, Fc)

    CD137; ILA; TNFRSF9; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA

    Human HEK293
    4-1BBR/TNFRSF9 Protein, Human (HEK 293, Fc), a recombinant human 4-1BBR/TNFRSF9 Protein produced in HEK293 cells, has an Fc fragment at the C-terminus. Recombinant Human 4-1BBRTNFRSF9, an inducible T cell molecule belonging to the TNF receptor superfamily, could promote the expansion of antigen-specific T cells and prevent activation-induced death of CD8+ T cells.
  • HY-P70778
    BCMA/TNFRSF17 Protein, Cynomolgus (HEK 293, His)

    Tumor necrosis factor receptor superfamily member 17; B-cell maturation protein; CD269; Tnfrsf17; Bcm; Bcma

    Cynomolgus HEK293
Cat. No. Product Name Effect Purity

Following the binding of TNF to TNF receptors, TNFR1 binds to TRADD, which recruits RIPK1, TRAF2/5 and cIAP1/2 to form TNFR1 signaling complex I; TNFR2 binds to TRAF1/2 directly to recruit cIAP1/2. Both cIAP1 and cIAP2 are E3 ubiquitin ligases that add K63 linked polyubiquitin chains to RIPK1 and other components of the signaling complex. The ubiquitin ligase activity of the cIAPs is needed to recruit the LUBAC, which adds M1 linked linear polyubiquitin chains to RIPK1. K63 polyubiquitylated RIPK1 recruits TAB2, TAB3 and TAK1, which activate signaling mediated by JNK and p38, as well as the IκB kinase complex. The IKK complex then activates NF-κB signaling, which leads to the transcription of anti-apoptotic factors-such as FLIP and Bcl-XL-that promote cell survival. 

 

The formation of TNFR1 complex IIa and complex IIb depends on non-ubiquitylated RIPK1. For the formation of complex IIa, ubiquitylated RIPK1 in complex I is deubiquitylated by CYLD. This deubiquitylated RIPK1 dissociates from the membrane-bound complex and moves into the cytosol, where it interacts with TRADD, FADD, Pro-caspase 8 and FLIPL to form complex IIa. By contrast, complex IIb is formed when the RIPK1 in complex I is not ubiquitylated owing to conditions that have resulted in the depletion of cIAPs, which normally ubiquitylate RIPK1. This non-ubiquitylated RIPK1 dissociates from complex I, moves into the cytosol, and assembles with FADD, Pro-caspase 8, FLIPL and RIPK3 (but not TRADD) to form complex IIb. For either complex IIa or complex IIb to prevent necroptosis, both RIPK1 and RIPK3 must be inactivated by the cleavage activity of the Pro-caspase 8-FLIPL heterodimer or fully activated caspase 8. The Pro-caspase 8 homodimer generates active Caspase 8, which is released from complex IIa and complex IIb. This active Caspase 8 then carries out cleavage reactions to activate downstream executioner caspases and thus induce classical apoptosis. 

 

Formation of the complex IIc (necrosome) is initiated either by RIPK1 deubiquitylation mediated by CYLD or by RIPK1 non-ubiquitylation due to depletion of cIAPs, similar to complex IIa and complex IIb formation. RIPK1 recruits numerous RIPK3 molecules. They come together to form amyloid microfilaments called necrosomes. Activated RIPK3 phosphorylates and recruits MLKL, eventually leading to the formation of a supramolecular protein complex at the plasma membrane and necroptosis [1][2].

 

Reference:
[1]. Brenner D, et al. Regulation of tumour necrosis factor signalling: live or let die.Nat Rev Immunol. 2015 Jun;15(6):362-74. 
[2]. Conrad M, et al. Regulated necrosis: disease relevance and therapeutic opportunities.Nat Rev Drug Discov. 2016 May;15(5):348-66.