BTK Protein, Human (P. pastoris, His)

BTK Protein, a key non-receptor tyrosine kinase, is essential for B lymphocyte development and signaling. Upon BCR activation, BTK initiates a cascade, phosphorylating PLCG2 and activating downstream pathways, influencing calcium mobilization and PKC activation. In innate and adaptive immunity, particularly TLR pathways, BTK regulates TLR9 activation, induces NF-kappa-B activity, and activates NLRP3 inflammasome assembly. Its multifaceted functions include phosphorylating GTF2I, participating in apoptosis regulation, and serving as a platform for diverse signaling proteins. BTK Protein, Human (P. pastoris, His) is the recombinant human-derived BTK protein, expressed by P. pastoris, with N-6*His labeled tag. The total length of BTK Protein, Human (P. pastoris, His) is 659 a.a., with molecular weight of 78.2 kDa.

BTK Protein, a non-receptor tyrosine kinase, is indispensable for B lymphocyte development, differentiation, and signaling. Upon antigen binding to the B-cell antigen receptor (BCR), BTK triggers a signaling cascade leading to B-cell activation, phosphorylating PLCG2 and initiating downstream pathways involving calcium mobilization and protein kinase C (PKC) activation. Serving as a platform for diverse signaling proteins, BTK is implicated in cytokine receptor signaling pathways and is crucial in innate and adaptive immunity, particularly within the Toll-like receptors (TLR) pathway. It plays a critical role in regulating TLR9 activation in splenic B-cells and induces the activity of NF-kappa-B, contributing to the transcriptional regulation of numerous genes. Additionally, BTK activates NLRP3 inflammasome assembly, phosphorylates transcription factor GTF2I, and is involved in apoptosis regulation, highlighting its multifaceted functions in immune response and cellular processes.

Human

P. pastoris

N-6*His

Q06187 (M1-S659)

695  [NCBI]

AAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVVPEKNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKKPLPPEPAAAPVSTSELKKVVALYDYMPMNANDLQLRKGDEYFILEESNLPWWRARDKNGQEGYIPSNYVTEAEDSIEMYEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKSTGDPQGVIRHYVVCSTPQSQYYLAEKHLFSTIPELINYHQHNSAGLISRLKYPVSQQNKNAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGKWRGQYDVAIKMIKEGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERPTFKILLSNILDVMDEES

78.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.