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  5. CD1D Protein, Human (HEK293, His)

CD1D Protein, Human (HEK293, His)

Cat. No.: HY-P74327
Handling Instructions

The CD1D protein, a key antigen-presenting molecule, binds self and non-self glycolipids, presenting them to T-cell receptors on natural killer T-cells. Partnering with B2M, it centrally orchestrates immune responses, and its interactions with MHC II emphasize its significance in the intricate network of immune system regulation. CD1D Protein, Human (HEK293, His) is the recombinant human-derived CD1D protein, expressed by HEK293, with C-His labeled tag. The total length of CD1D Protein, Human (HEK293, His) is 301 a.a., with molecular weight of 45-50 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

The CD1D protein, a key antigen-presenting molecule, binds self and non-self glycolipids, presenting them to T-cell receptors on natural killer T-cells. Partnering with B2M, it centrally orchestrates immune responses, and its interactions with MHC II emphasize its significance in the intricate network of immune system regulation. CD1D Protein, Human (HEK293, His) is the recombinant human-derived CD1D protein, expressed by HEK293, with C-His labeled tag. The total length of CD1D Protein, Human (HEK293, His) is 301 a.a., with molecular weight of 45-50 kDa.

Background

The CD1D-B2M heterodimer protein is a crucial antigen-presenting molecule that exhibits the unique ability to bind both self and non-self glycolipids, facilitating their presentation to T-cell receptors on natural killer T-cells. Forming a heterodimeric complex with B2M (beta-2-microglobulin), this protein plays a central role in orchestrating immune responses. Its interactions with MHC II further contribute to its significance in the intricate network of immune system regulation.

Species

Human

Source

HEK293

Tag

C-His

Accession

P15813 (M1-S301)

Gene ID

912  [NCBI]

Molecular Construction
N-term
CD1D (M1-S301)
Accession # P15813
His
C-term
Synonyms
Antigen-presenting glycoprotein CD1d; CD1D; R3G1
Molecular Weight

45-50 kDa

Purity

Greater than 88% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CD1D Protein, Human (HEK293, His)
Cat. No.:
HY-P74327
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