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  5. CDC42BPB Protein, Human (sf9, His-GST)

CDC42BPB Protein, Human (sf9, His-GST)

Cat. No.: HY-P76815
Handling Instructions

CDC42BPB protein is a key serine/threonine protein kinase downstream of CDC42 that controls cytoskeletal dynamics and cell migration. CDC42BPB phosphorylates PPP1R12C and MYL9/MLC2, regulates actin reorganization and shapes cell dynamics. CDC42BPB Protein, Human (sf9, His-GST) is the recombinant human-derived CDC42BPB protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of CDC42BPB Protein, Human (sf9, His-GST) is 427 a.a., with molecular weight of ~70 kDa.

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Description

CDC42BPB protein is a key serine/threonine protein kinase downstream of CDC42 that controls cytoskeletal dynamics and cell migration. CDC42BPB phosphorylates PPP1R12C and MYL9/MLC2, regulates actin reorganization and shapes cell dynamics. CDC42BPB Protein, Human (sf9, His-GST) is the recombinant human-derived CDC42BPB protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of CDC42BPB Protein, Human (sf9, His-GST) is 427 a.a., with molecular weight of ~70 kDa.

Background

CDC42BPB Protein, a serine/threonine-protein kinase, stands as a pivotal downstream effector of CDC42, exerting significant influence over cytoskeleton reorganization and cell migration. Through the phosphorylation of PPP1R12C and MYL9/MLC2, CDC42BPB orchestrates the regulation of actin cytoskeletal reorganization, emphasizing its role in shaping cellular dynamics. Collaborating with MYO18A and LURAP1, CDC42BPB actively participates in the modulation of lamellar actomyosin retrograde flow, a process critical for cell protrusion and migration. Furthermore, CDC42BPB phosphorylates PPP1R12A, contributing to its multifaceted impact on cellular functions. In tandem with FAM89B/LRAP25, CDC42BPB facilitates the targeting of LIMK1 to the lamellipodium, initiating its activation and subsequent phosphorylation of CFL1, a crucial event for the regulation of lamellipodial F-actin dynamics.

Species

Human

Source

Sf9 insect cells

Tag

N-His;N-GST

Accession

Q9Y5S2 (M1-H427)

Gene ID
Molecular Construction
N-term
His-GST
CDC42BPB (M1-H427)
Accession # Q9Y5S2
C-term
Synonyms
Serine/threonine-protein kinase MRCK beta; CDC42BP-beta; DMPK-like beta; MRCK beta
Molecular Weight

Approximately 70 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

CDC42BPB Protein, Human (sf9, His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CDC42BPB Protein, Human (sf9, His-GST)
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HY-P76815
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