cIAP1/HIAP-2 Protein, Human (His)

The multifunctional cIAP1/HIAP-2 protein serves as a key coordinator affecting caspases, apoptosis, inflammatory signaling, immunity, mitotic kinase signaling, cell proliferation, invasion, and metastasis. As an E3 ubiquitin protein ligase, it complexly regulates NF-kappa-B signaling, acting as both a positive regulator of the classical pathway and a suppressor of non-canonical NF-kappa-B signaling. cIAP1/HIAP-2 Protein, Human (His) is the recombinant human-derived cIAP1/HIAP-2 protein, expressed by E. coli , with C-His labeled tag. The total length of cIAP1/HIAP-2 Protein, Human (His) is 213 a.a., with molecular weight of ~26 kDa.

The cIAP1/HIAP-2 protein emerges as a multi-functional orchestrator governing diverse cellular processes, encompassing caspases and apoptosis regulation, modulation of inflammatory signaling and immunity, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis. Functioning as an E3 ubiquitin-protein ligase, cIAP1/HIAP-2 intricately regulates NF-kappa-B signaling, acting both as a positive regulator of the canonical pathway and a suppressor of constitutive activation in the non-canonical NF-kappa-B signaling. Targeting a spectrum of proteins for ubiquitination, including RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO, IKBKE, and MXD1/MAD1, cIAP1/HIAP-2 also functions as an E3 ubiquitin-protein ligase in the NEDD8 conjugation pathway, impacting effector caspases. As a pivotal regulator of innate immune signaling through pattern recognition receptors (PRRs), it shields cells from the spontaneous formation of the ripoptosome—a multi-protein complex capable of inducing caspase-dependent and caspase-independent cell death—by ubiquitinating RIPK1 and CASP8. Moreover, cIAP1/HIAP-2 can stimulate the transcriptional activity of E2F1 and plays a role in modulating the cell cycle, illustrating its central role in the intricate landscape of cellular processes.

Human

E. coli

C-His

Q13490 (E144-L356)

329  [NCBI]

Approximately 26 kDa

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.