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  4. ERK2 Protein, Mouse (sf9, His-GST)

ERK2 Protein, Mouse (sf9, His-GST)

Cat. No.: HY-P74167
Handling Instructions

ERK2 protein is an essential serine/threonine kinase in the MAP kinase pathway and cooperates with MAPK1/ERK2 in the complex MAPK/ERK cascade. Stimulated by KIT and KITLG/SCF, this pathway is central to cellular function, affecting growth, adhesion, survival, and differentiation through transcription, translation, and cytoskeletal regulation. ERK2 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived ERK2 protein, expressed by Sf9 insect cells , with N-GST, N-His labeled tag. The total length of ERK2 Protein, Mouse (sf9, His-GST) is 358 a.a., with molecular weight of ~60 kDa.

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Description

ERK2 protein is an essential serine/threonine kinase in the MAP kinase pathway and cooperates with MAPK1/ERK2 in the complex MAPK/ERK cascade. Stimulated by KIT and KITLG/SCF, this pathway is central to cellular function, affecting growth, adhesion, survival, and differentiation through transcription, translation, and cytoskeletal regulation. ERK2 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived ERK2 protein, expressed by Sf9 insect cells , with N-GST, N-His labeled tag. The total length of ERK2 Protein, Mouse (sf9, His-GST) is 358 a.a., with molecular weight of ~60 kDa.

Background

ERK2, a serine/threonine kinase and indispensable component of the MAP kinase signal transduction pathway, collaborates with MAPK1/ERK2 in orchestrating the intricate MAPK/ERK cascade. Activated by stimuli such as KIT and KITLG/SCF, this pathway assumes a central role in diverse cellular functions, including cell growth, adhesion, survival, and differentiation, achieved through the regulation of transcription, translation, and cytoskeletal rearrangements. ERK2 further contributes to the initiation and regulation of meiosis, mitosis, and postmitotic functions by phosphorylating numerous transcription factors. With approximately 160 substrates identified, ERK2 impacts cellular processes in the nucleus, cytosol, and various cellular organelles, influencing transcription, translation, mitosis, and apoptosis. Additionally, the MAPK/ERK cascade, facilitated by ERK2, is implicated in endosomal dynamics, lysosome processing, endosome cycling through the perinuclear recycling compartment (PNRC), and Golgi apparatus fragmentation during mitosis. Substrates of ERK2 span transcription factors, cytoskeletal elements, regulators of apoptosis, regulators of translation, and diverse signaling-related molecules, as well as protein kinases and phosphatases. Notably, ERK2 mediates phosphorylation events, such as those on TPR in response to EGF stimulation, and plays a role in the spindle assembly checkpoint. It phosphorylates PML, promoting its interaction with PIN1 and subsequent degradation, and phosphorylates CDK2AP2. Acting as a transcriptional repressor, ERK2 binds to specific consensus sequences and represses the expression of interferon gamma-induced genes, demonstrating its multifaceted regulatory role beyond its kinase activity.

Species

Mouse

Source

Sf9 insect cells

Tag

N-GST;N-His

Accession

P63085 (M1-S358)

Gene ID
Molecular Construction
N-term
His-GST
ERK2 (M1-S358)
Accession # P63085
C-term
Synonyms
Mitogen-activated protein kinase 1; MAPK 1; ERT1; ERK-2; p42-MAPK
Molecular Weight

Approximately 60 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

ERK2 Protein, Mouse (sf9, His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ERK2 Protein, Mouse (sf9, His-GST)
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HY-P74167
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