1. Recombinant Proteins
  2. Fc Receptor Proteins
  3. Fc Receptor binding IgG
  4. FcγRII/CD32
  5. Fc gamma RIIA/CD32a Protein, Human (210a.a, HEK293, His-Avi)

Fc gamma RIIA/CD32a Protein, Human (210a.a, HEK293, His-Avi)

Cat. No.: HY-P76231
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Fc gamma RIIA/CD32a Protein, Human (210a.a, HEK293, His-Avi) Related Classifications

  • Technical Parameters

  • Properties

  • Documentation

Species

Human

Source

HEK 293

Tag

His-Avi

Accession

P12318 (M1-M210)

Gene ID

2212

Synonyms
C-X-C motif chemokine 12; Stromal cell-derived factor 1; CXCL12; SDF-1β
AA Sequence

Molecular Weight

Approximately 32 kDa.

Purity

Greater than 98 % as determined by SDS-PAGE

Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized human CD32a-Avi at 10 μg/mL (100 μL/well) can bind biotinylated human IgG1 and the EC50 is 0.13-0.29 μg/mL. Using the Octet RED System, the affinity constant (Kd) of human CD32A
Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
  • Reconstitution Calculator

  • Dilution Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Fc gamma RIIA/CD32a Protein, Human (210a.a, HEK293, His-Avi)
Cat. No.:
HY-P76231
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