1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Peptide Hormone & Neuropeptides
  4. Growth Hormone/Somatotropin
  5. GH1/Somatotropin Protein, Human (HEK293, N-His, C-Myc)

GH1/Somatotropin Protein, Human (HEK293, N-His, C-Myc)

Cat. No.: HY-P700412
COA Handling Instructions

Growth hormone (GH) plays a key role in growth control, stimulating the release of insulin-like growth factor 1 (IGF-1) from the liver and tissues to promote body growth. It regulates myoblast differentiation and proliferation and contributes significantly to overall organismal development. GH1/Somatotropin Protein, Human (HEK293, N-His, C-Myc) is the recombinant human-derived GH1/Somatotropin protein, expressed by HEK293 , with C-Myc, N-10*His labeled tag. The total length of GH1/Somatotropin Protein, Human (HEK293, N-His, C-Myc) is 191 a.a., with molecular weight of 27.2 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

Growth hormone (GH) plays a key role in growth control, stimulating the release of insulin-like growth factor 1 (IGF-1) from the liver and tissues to promote body growth. It regulates myoblast differentiation and proliferation and contributes significantly to overall organismal development. GH1/Somatotropin Protein, Human (HEK293, N-His, C-Myc) is the recombinant human-derived GH1/Somatotropin protein, expressed by HEK293 , with C-Myc, N-10*His labeled tag. The total length of GH1/Somatotropin Protein, Human (HEK293, N-His, C-Myc) is 191 a.a., with molecular weight of 27.2 kDa.

Background

The Somatotropin (GH) protein plays a pivotal role in growth control, exerting its primary influence on body growth by stimulating the liver and other tissues to secrete insulin-like growth factor 1 (IGF-1). GH serves as a potent regulator of both the differentiation and proliferation of myoblasts, contributing significantly to the overall growth and development of the organism. Additionally, it plays a crucial role in enhancing amino acid uptake and promoting protein synthesis in muscle and various tissues. Structurally, GH exists in various forms, including monomers, dimers, trimers, tetramers, and pentamers, either disulfide-linked or non-covalently associated, in homomeric and heteromeric combinations. Furthermore, GH can form complexes with GH binding protein (GHBP) or with the alpha2-macroglobulin complex, underscoring its versatile molecular interactions that contribute to its multifaceted roles in growth regulation and tissue development.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized GH1 at 1 μg/mL can bind human GHR, the EC50 of the protein is ≤25.29 ng/ml.

Species

Human

Source

HEK293

Tag

C-Myc;N-10*His

Accession

P01241 (F27-F217)

Gene ID
Molecular Construction
N-term
10*His-Myc
GH (F27-F217)
Accession # P01241
C-term
Synonyms
GH; GH-N; GHN; hGH-N; Growth hormone; Pituitary growth hormone; growth hormone 1; GH1; IGHD1B; Somatotropin; hGH
AA Sequence

FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF

Molecular Weight

27.2 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

GH1/Somatotropin Protein, Human (HEK293, N-His, C-Myc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GH1/Somatotropin Protein, Human (HEK293, N-His, C-Myc)
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HY-P700412
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