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Gliomedin Protein, Human (HEK293, Fc)

Cat. No.: HY-P76363
COA Handling Instructions

Gliomedin Protein, a collagen-rich homotrimer, crucially binds NRCAM and NFASC, playing a pivotal role in establishing and preserving nodes of Ranvier on myelinated axons. It mediates Schwann cell-axon interaction, promoting sodium channel clustering for efficient action potential propagation. Gliomedin, collaborating with NRCAM, ensures proper sodium channel clustering during development and maintains NFASC and sodium channel clusters at mature nodes of Ranvier. Its intricate involvement underscores Gliomedin Protein's significance in the structural integrity and organization of nodes of Ranvier. Gliomedin Protein, Human (HEK293, Fc) is the recombinant human-derived Gliomedin protein, expressed by HEK293 , with N-hFc labeled tag and with Natural variant S141N mutation. The total length of Gliomedin Protein, Human (HEK293, Fc) is 427 a.a., with molecular weight of ~74.3 KDa.

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Description

Gliomedin Protein, a collagen-rich homotrimer, crucially binds NRCAM and NFASC, playing a pivotal role in establishing and preserving nodes of Ranvier on myelinated axons. It mediates Schwann cell-axon interaction, promoting sodium channel clustering for efficient action potential propagation. Gliomedin, collaborating with NRCAM, ensures proper sodium channel clustering during development and maintains NFASC and sodium channel clusters at mature nodes of Ranvier. Its intricate involvement underscores Gliomedin Protein's significance in the structural integrity and organization of nodes of Ranvier. Gliomedin Protein, Human (HEK293, Fc) is the recombinant human-derived Gliomedin protein, expressed by HEK293 , with N-hFc labeled tag and with Natural variant S141N mutation. The total length of Gliomedin Protein, Human (HEK293, Fc) is 427 a.a., with molecular weight of ~74.3 KDa.

Background

Gliomedin, a homotrimeric protein characterized by collagen-like domains, acts as a crucial ligand for NRCAM and NFASC/neurofascin, playing a pivotal role in the establishment and preservation of nodes of Ranvier along myelinated axons. Its significance lies in mediating the interaction between Schwann cell microvilli and axons through binding to NRCAM and NFASC. Nodes of Ranvier are essential regions on myelinated axons housing clustered sodium channels vital for the saltatory propagation of action potentials. Gliomedin is specifically involved in the formation of nodes during development by facilitating the fusion of heminodes, and it is indispensable for the proper clustering of sodium channels at heminodes. Additionally, in collaboration with NRCAM, Gliomedin contributes to the maintenance of NFASC and sodium channel clusters at fully mature nodes of Ranvier. Furthermore, its interaction with glial NRCAM enhances its binding affinity with axonal NFASC. Gliomedin's intricate involvement underscores its significance in the structural integrity and functional organization of nodes of Ranvier.

Species

Human

Source

HEK293

Tag

N-hFc

Accession

Q6ZMI3-2 (M1-Q427, with Natural variant S141N)

Gene ID
Molecular Construction
N-term
hFc
Gliomedin (M1-Q427, with Natural variant S141N)
Accession # Q6ZMI3-2
C-term
Synonyms
Gliomedin; GLDN; COLM
Molecular Weight

Approximately 74.3 kDa.

Purity

Greater than 85% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Gliomedin Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Gliomedin Protein, Human (HEK293, Fc)
Cat. No.:
HY-P76363
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