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  3. Hydrolases (EC 3)
  4. Granzyme B/GZMB Protein, Mouse (CHO)

Granzyme B/GZMB Protein, Mouse (CHO)

Cat. No.: HY-P7185
COA Handling Instructions

Granzyme B/GZMB Protein, Mouse (CHO) plays essential roles in apoptosis and is involved in inducing inflammation by stimulating cytokine release and is also involved in extracellular matrix remodeling.

For research use only. We do not sell to patients.

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10 μg $210 In-stock
50 μg $620 In-stock
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  • Biological Activity

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Description

Granzyme B/GZMB Protein, Mouse (CHO) plays essential roles in apoptosis and is involved in inducing inflammation by stimulating cytokine release and is also involved in extracellular matrix remodeling.

Background

Granzyme B plays essential roles in mammalian apoptosis[1]. Granzyme B is a serine protease most commonly found in the granules of cytotoxic lymphocytes (CTLs), natural killer cells (NK cells) and cytotoxic T cells. It is secreted by these cells along with the pore forming protein perforin to mediate apoptosis in target cells. Granzyme B has shown to be involved in inducing inflammation by stimulating cytokine release and is also involved in extracellular matrix remodeling[2].

Biological Activity

Measured by its ability to cleave a peptide substrate, t-Butyloxycaronyl-Ala-Ala-Asp-ThioBenzyl ester (Boc-AAD-SBzl), in the presence of 5,5’-Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is 3299 pmol/min/µg as measured under the described conditions.

Species

Mouse

Source

CHO

Tag

Tag Free

Accession

P04187 (I21-S247)

Gene ID

14939  [NCBI]

Molecular Construction
N-term
GZMB (I21-S247)
Accession # P04187
C-term
Synonyms
rMuGranzyme B; CTLA-1; CCP1; Fragmentin-2; Gzmb
AA Sequence

IIGGHEVKPHSRPYMALLSIKDQQPEAICGGFLIREDFVLTAAHCEGSIINVTLGAHNIKEQEKTQQVIPMVKCIPHPDYNPKTFSNDIMLLKLKSKAKRTRAVRPLNLPRRNVNVKPGDVCYVAGWGRMAPMGKYSNTLQEVELTVQKDRECESYFKNRYNKTNQICAGDPKTKRASFRGDSGGPLVCKKVAAGIVSYGYKDGSPPRAFTKVSSFLSWIKKTMKSS

Molecular Weight

Approximately 32 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

Granzyme B/GZMB Protein, Mouse (CHO) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Granzyme B/GZMB Protein, Mouse (CHO)
Cat. No.:
HY-P7185
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