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  4. Granzyme B/GZMB Protein, Mouse (HEK293, His)

Granzyme B/GZMB Protein, Mouse (HEK293, His)

Cat. No.: HY-P70253
COA Handling Instructions

Granzyme B/GZMB Protein, found in cytotoxic T-cells and NK-cells, activates caspase-independent pyroptosis and cleaves gasdermin-E (GSDME), inducing target cell death. It also participates in caspase activation cascade, promoting apoptosis and plasma membrane repair during bacterial infection. Granzyme B/GZMB Protein, Mouse (HEK293, His) is the recombinant mouse-derived Granzyme B/GZMB protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Granzyme B/GZMB Protein, Mouse (HEK293, His) is 229 a.a., with molecular weight of ~35.0 kDa.

For research use only. We do not sell to patients.

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Description

Granzyme B/GZMB Protein, found in cytotoxic T-cells and NK-cells, activates caspase-independent pyroptosis and cleaves gasdermin-E (GSDME), inducing target cell death. It also participates in caspase activation cascade, promoting apoptosis and plasma membrane repair during bacterial infection. Granzyme B/GZMB Protein, Mouse (HEK293, His) is the recombinant mouse-derived Granzyme B/GZMB protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Granzyme B/GZMB Protein, Mouse (HEK293, His) is 229 a.a., with molecular weight of ~35.0 kDa.

Background

Granzyme B/GZMB Protein, an abundant protease found in the cytosolic granules of cytotoxic T-cells and NK-cells, plays a crucial role in various cellular processes. When delivered into the target cell through the immunological synapse, Granzyme B/GZMB activates caspase-independent pyroptosis, leading to target cell death. It achieves this by cleaving after Asp and catalyzing the cleavage of gasdermin-E (GSDME), releasing the pore-forming component of GSDME, which triggers pyroptosis. Granzyme B/GZMB is also involved in the activation cascade of caspases, including caspase-3, -9, and -7, which are responsible for apoptosis execution and plasma membrane repair in response to bacterial infection.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Mouse

Source

HEK293

Tag

C-6*His

Accession

P04187 (G19-S247)

Gene ID

14939  [NCBI]

Molecular Construction
N-term
GZMB (G19-S247)
Accession # P04187
6*His
C-term
Synonyms
rMuGranzyme B/GZMB, His; Granzyme B(G; H); CTLA-1; Cytotoxic cell protease 1; CCP1; Fragmentin-2; Gzmb; Ctla-1; Ctla1
AA Sequence

GEIIGGHEVKPHSRPYMALLSIKDQQPEAICGGFLIREDFVLTAAHCEGSIINVTLGAHNIKEQEKTQQVIPMVKCIPHPDYNPKTFSNDIMLLKLKSKAKRTRAVRPLNLPRRNVNVKPGDVCYVAGWGRMAPMGKYSNTLQEVELTVQKDRECESYFKNRYNKTNQICAGDPKTKRASFRGDSGGPLVCKKVAAGIVSYGYKDGSPPRAFTKVSSFLSWIKKTMKSS

Molecular Weight

Approximately 35.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Granzyme B/GZMB Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Granzyme B/GZMB Protein, Mouse (HEK293, His)
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