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  3. GRIN1 Protein, Human (His)

GRIN1 Protein, Human (His)

Cat. No.: HY-P72213
COA Handling Instructions

The GRIN1 protein is a component of the NMDA receptor complex that forms heterotetrameric ligand-gated ion channels with high calcium permeability. GRIN1 Protein, Human (His) is the recombinant human-derived GRIN1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of GRIN1 Protein, Human (His) is 541 a.a., with molecular weight of ~64.6 kDa.

For research use only. We do not sell to patients.

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Description

The GRIN1 protein is a component of the NMDA receptor complex that forms heterotetrameric ligand-gated ion channels with high calcium permeability. GRIN1 Protein, Human (His) is the recombinant human-derived GRIN1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of GRIN1 Protein, Human (His) is 541 a.a., with molecular weight of ~64.6 kDa.

Background

GRIN1, a pivotal component of NMDA receptor complexes, participates in the constitution of heterotetrameric ligand-gated ion channels that exhibit both high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation hinges on the intricate interplay of neurotransmitter binding, with glutamate binding to the epsilon subunit and glycine binding to the zeta subunit, combined with membrane depolarization to alleviate channel inhibition by Mg(2+). The sensitivity to glutamate, as well as the kinetics of the channel, is contingent upon the specific subunit composition. GRIN1 forms heterotetrameric channels typically comprised of two zeta subunits and two epsilon subunits, where the latter can be either GRIN2A, GRIN2B, GRIN2C, or GRIN2D. Additionally, it can participate in the formation of channels with GRIN3A or GRIN3B. The dynamic subunit composition in vivo further underscores the regulatory intricacies of GRIN1's involvement in NMDA receptor function. Furthermore, GRIN1 engages in various protein interactions, such as those with SNX27, DLG4, MPDZ, LRFN1, LRFN2, MYZAP, and PRR7, contributing to its multifaceted role within cellular processes.

Species

Human

Source

E. coli

Tag

N-6*His

Accession

Q05586 (R19-Q559)

Gene ID
Molecular Construction
N-term
6*His
GRIN1 (R19-Q559)
Accession # Q05586
C-term
Synonyms
GluN1; Glutamate [NMDA] receptor subunit zeta-1; Glutamate receptor ionotropic N methyl D aspartate 1; glutamate receptor ionotropic, NMDA 1; Grin1; MRD8
AA Sequence

RAACDPKIVNIGAVLSTRKHEQMFREAVNQANKRHGSWKIQLNATSVTHKPNAIQMALSVCEDLISSQVYAILVSHPPTPNDHFTPTPVSYTAGFYRIPVLGLTTRMSIYSDKSIHLSFLRTVPPYSHQSSVWFEMMRVYSWNHIILLVSDDHEGRAAQKRLETLLEERESKAEKVLQFDPGTKNVTALLMEAKELEARVIILSASEDDAATVYRAAAMLNMTGSGYVWLVGEREISGNALRYAPDGILGLQLINGKNESAHISDAVGVVAQAVHELLEKENITDPPRGCVGNTNIWKTGPLFKRVLMSSKYADGVTGRVEFNEDGDRKFANYSIMNLQNRKLVQVGIYNGTHVIPNDRKIIWPGGETEKPRGYQMSTRLKIVTIHQEPFVYVKPTLSDGTCKEEFTVNGDPVKKVICTGPNDTSPGSPRHTVPQCCYGFCIDLLIKLARTMNFTYEVHLVADGKFGTQERVNNSNKKEWNGMMGELLSGQADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQ

Molecular Weight

Approximately 64.6 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

GRIN1 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GRIN1 Protein, Human (His)
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