1. Recombinant Proteins
  2. Immune Checkpoint Proteins
  3. Inhibitory Checkpoint Molecules
  4. IDO
  5. IDO Protein, Human

IDO Protein, Human

Cat. No.: HY-P73121
Handling Instructions

IDO proteins catalyze the first step in tryptophan catabolism via the kynurenine pathway, which is critical for immune tolerance and homeostasis maintenance. IDO Protein, Human is the recombinant human-derived IDO protein, expressed by E. coli , with tag free. The total length of IDO Protein, Human is 402 a.a., with molecular weight of ~46 kDa.

For research use only. We do not sell to patients.

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Description

IDO proteins catalyze the first step in tryptophan catabolism via the kynurenine pathway, which is critical for immune tolerance and homeostasis maintenance. IDO Protein, Human is the recombinant human-derived IDO protein, expressed by E. coli , with tag free. The total length of IDO Protein, Human is 402 a.a., with molecular weight of ~46 kDa.

Background

The IDO protein plays a central role in cellular processes by catalyzing the initial and rate-limiting step in the catabolism of the essential amino acid tryptophan along the kynurenine pathway, as documented in various studies. Beyond its enzymatic function, IDO is involved in peripheral immune tolerance, contributing to the maintenance of homeostasis by preventing autoimmunity or immunopathology resulting from uncontrolled and overreacting immune responses. Tryptophan scarcity, a consequence of IDO activity, inhibits T lymphocytes division, and the accumulation of tryptophan catabolites induces T-cell apoptosis while promoting the differentiation of regulatory T-cells. Additionally, IDO acts as a suppressor of anti-tumor immunity and limits the growth of intracellular pathogens by depriving them of tryptophan. Furthermore, IDO serves a crucial role in protecting the fetus from maternal immune rejection. These diverse functions underscore the significance of IDO in orchestrating immune responses, maintaining metabolic balance, and contributing to crucial physiological processes with implications for autoimmunity, cancer, and host-pathogen interactions.

Species

Human

Source

E. coli

Tag

Tag Free

Accession

P14902 (A2-G403)

Gene ID
Molecular Construction
N-term
IDO (A2-G403)
Accession # P14902
C-term
Synonyms
Indoleamine 2; 3-dioxygenase 1; IDO-1; IDO1; IDO; INDO
Molecular Weight

Approximately 46 kDa

Purity

Greater than 85% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

IDO Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IDO Protein, Human
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HY-P73121
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