1. Recombinant Proteins
  2. IL-4, Human (HEK293, His)

IL-4, Human (HEK293, His)

Cat. No.: HY-P70764
Handling Instructions

For research use only. We do not sell to patients.

Protein Expression Service

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  • Technical Parameters

  • Properties

  • Documentation

Synonyms
Interleukin-4; IL-4; B-Cell Stimulatory Factor 1; BSF-1; Binetrakin; Lymphocyte Stimulatory Factor 1; Pitrakinra; IL4
Species

Human

Source

HEK 293

Accession

P05112

Gene ID

3565

Molecular Weight

Approximately 18.0 kDa

AA Sequence

HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Biological Activity
The cell proliferation assay using TF-1 human erythroleukemic cells has an ED50 value of 0.05-0.2 ng/mL. Human IL-4-His can bind Human IL-4RA-Fc with an affinity constant of 0.12 nM.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Reconstitute at 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
IL-4, Human (HEK293, His)
Cat. No.:
HY-P70764
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