1. Recombinant Proteins
  2. Receptor Proteins
  3. Leptin R/LEPR Protein, Human (839a.a, HEK293, His)

Leptin R/LEPR Protein, Human (839a.a, HEK293, His)

Cat. No.: HY-P73274
Handling Instructions

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized human Leptin at 5 μg/mL (100 μl/well) can bind Leptin R/LEPR Protein, Human (839a.a, HEK293, His) with a linear range of 0.032-4.0 μg/mL.
Species

Human

Source

HEK293

Tag

C-His

Accession

P48357 (M1-D839)

Gene ID
Synonyms
Leptin receptor; LEP-R; HuB219; OB-R; CD295; DB
Molecular Weight

130-140 kDa

Purity

Greater than 95 % as determined by SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Leptin R/LEPR Protein, Human (839a.a, HEK293, His) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Leptin R/LEPR Protein, Human (839a.a, HEK293, His)
Cat. No.:
HY-P73274
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