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  4. MCEE Protein, Human (HEK293, His)

MCEE Protein, Human (HEK293, His)

Cat. No.: HY-P70895
Handling Instructions

MCEE Protein, a methylmalonyl-CoA epimerase, crucially participates in propionyl-CoA metabolism. MCEE Protein, Human (HEK293, His) is the recombinant human-derived MCEE protein, expressed by HEK293 , with C-6*His labeled tag. The total length of MCEE Protein, Human (HEK293, His) is 140 a.a., with molecular weight of 18-20 kDa.

For research use only. We do not sell to patients.

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Description

MCEE Protein, a methylmalonyl-CoA epimerase, crucially participates in propionyl-CoA metabolism. MCEE Protein, Human (HEK293, His) is the recombinant human-derived MCEE protein, expressed by HEK293 , with C-6*His labeled tag. The total length of MCEE Protein, Human (HEK293, His) is 140 a.a., with molecular weight of 18-20 kDa.
Background

MCEE protein, functioning as a methylmalonyl-CoA epimerase, plays a crucial role in propionyl-CoA metabolism.
Species

Human
Source

HEK293
Tag

C-6*His
Accession

Q96PE7 (Q37-A176)
Gene ID
Molecular Construction
N-term
MCEE (Q37-A176)
Accession # Q96PE7
6*His
C-term
Synonyms
Methylmalonyl-CoA epimerase; mitochondrial; DL-methylmalonyl-CoA racemase
AA Sequence

QVTGSVWNLGRLNHVAIAVPDLEKAAAFYKNILGAQVSEAVPLPEHGVSVVFVNLGNTKMELLHPLGRDSPIAGFLQKNKAGGMHHICIEVDNINAAVMDLKKKKIRSLSEEVKIGAHGKPVIFLHPKDCGGVLVELEQA

Molecular Weight

18-20 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

MCEE Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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