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  4. MEK1 Protein, Mouse (sf9)

MEK1 Protein, Mouse (sf9)

Cat. No.: HY-P73826
Handling Instructions

The MEK1 protein is a dual-specificity kinase that plays a crucial role in the MAP kinase pathway. MEK1 regulates various cellular processes such as growth, adhesion, survival, and differentiation, as well as transcription, metabolism, and cytoskeletal rearrangements. MEK1 Protein, Mouse (sf9) is the recombinant mouse-derived MEK1 protein, expressed by Sf9 insect cells , with tag free. The total length of MEK1 Protein, Mouse (sf9) is 393 a.a., with molecular weight of ~45 kDa.

For research use only. We do not sell to patients.

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Description

The MEK1 protein is a dual-specificity kinase that plays a crucial role in the MAP kinase pathway. MEK1 regulates various cellular processes such as growth, adhesion, survival, and differentiation, as well as transcription, metabolism, and cytoskeletal rearrangements. MEK1 Protein, Mouse (sf9) is the recombinant mouse-derived MEK1 protein, expressed by Sf9 insect cells , with tag free. The total length of MEK1 Protein, Mouse (sf9) is 393 a.a., with molecular weight of ~45 kDa.

Background

MEK1 protein, a pivotal component of the MAP kinase signal transduction pathway, plays a crucial role in transducing extracellular signals into intracellular responses. Upon binding of ligands such as growth factors and cytokines to their cell-surface receptors, MEK1 is activated as part of the RAS-RAF1-MEK1/2-MAPK/ERK cascade. MEK1, along with MAP2K2/MEK2, catalyzes the dual phosphorylation of threonine and tyrosine residues in the activation loop of ERK1 and ERK2, thereby initiating downstream signaling events. This cascade regulates diverse biological functions, including cell growth, adhesion, survival, and differentiation. In addition to its role in the transcriptional and cytoskeletal regulation, the MAPK/ERK pathway impacts peroxisome proliferator-activated receptor gamma (PPARG), contributing to cellular processes such as differentiation and apoptosis. Furthermore, MEK1's involvement in endosomal dynamics and Golgi apparatus fragmentation during mitosis underscores its versatility in coordinating intricate cellular responses.

Species

Mouse

Source

Sf9 insect cells

Tag

Tag Free

Accession

P31938 (N-G&P, M1-I393)

Gene ID
Molecular Construction
N-term
Gly-Pro
Gly-Pro
MEK1 (M1-I393)
Accession # P31938
C-term
Synonyms
Dual specificity mitogen-activated protein kinase kinase 1; MAPKK 1; MEK 1; Map2k1
Molecular Weight

Approximately 45 kDa

Purity

Greater than 85% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

MEK1 Protein, Mouse (sf9) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MEK1 Protein, Mouse (sf9)
Cat. No.:
HY-P73826
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