MKK4 Protein, Mouse (sf9, His-GST)

The MKK4 protein is a dual-specificity kinase involved in the MAP kinase pathway. It activates stress-activated protein kinase (JNK) via phosphorylation with a preference for Thr or Tyr residues. MKK4 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived MKK4 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of MKK4 Protein, Mouse (sf9, His-GST) is 396 a.a., with molecular weight of ~65 kDa.

MKK4, a dual specificity protein kinase, plays a crucial role as an essential component in the MAP kinase signal transduction pathway, specifically in the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling cascade. Alongside MAP2K7/MKK7, MKK4 is one of the few known kinases that directly activate stress-activated protein kinases, including MAPK8/JNK1, MAPK9/JNK2, and MAPK10/JNK3, by phosphorylating the Thr-Pro-Tyr motif. While both MKK4 and MAP2K7/MKK7 contribute to JNK activation through phosphorylation, they exhibit distinct preferences for phosphorylation sites within this motif. MKK4 shows a preference for phosphorylating the Tyr residue, whereas MAP2K7/MKK7 prefers the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 appears to be a prerequisite for JNK activation, particularly in response to pro-inflammatory cytokines. Interestingly, other stimuli activate both MKK4 and MAP2K7/MKK7, working synergistically to phosphorylate JNKs. MKK4's involvement extends to the maintenance of peripheral lymphoid homeostasis and participation in the mitochondrial death signaling pathway, leading to apoptosis. Notably, while MAP2K7/MKK7 exclusively activates JNKs, MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13, and MAPK14.

Mouse

Sf9 insect cells

N-His;N-GST

P47809 (A2-D397)

26398  [NCBI]

Approximately 65 kDa

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.