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  5. PD-1 Protein, Human (167a.a, HEK293, His)

PD-1 Protein, Human (167a.a, HEK293, His)

Cat. No.: HY-P73346
Handling Instructions
Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized human PD-1 at 10 μg/mL (100 μL/well) can bind human B7-H1/PD-L1 (Fc) chimera with a linear range of 0.02-0.4 μg/mL. Labeled biotin to PD-1 Protein, Human (His) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of human PD-1 Protein bound to Keytruda was 4.7 nM.







Q15116 (M1-Q167)

Gene ID
CD279; hPD-1; PDCD1; Programmed cell death 1; SLEB2
Molecular Weight

Approximately 41.1 kDa


Greater than 95 % as determined by SDS-PAGE


Lyophilized powder.


Lyophilized a 0.2 μm filtered solution of 50 mM MOPS, 500 mM NaCl, pH 7.0

Endotoxin Level

<1 EU/μg, determined by LAL method.


It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.


Room temperature in continental US; may vary elsewhere.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PD-1 Protein, Human (167a.a, HEK293, His)
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