1. Recombinant Proteins
  2. Immune Checkpoint Proteins CD Antigens Receptor Proteins Biotinylated Proteins
  3. Inhibitory checkpoint molecules Stimulatory immune checkpoint molecules NK Cell CD Proteins Macrophage CD Proteins Monocyte CD Proteins Cell Adhesion Molecules (CAMs)
  4. Immunoglobulin-like Cell Adhesion Molecules
  5. PVR/CD155
  6. PVR/CD155 Protein, Human (Biotinylated, HEK293, His)

PVR/CD155 Protein, Human (Biotinylated, HEK293, His)

Cat. No.: HY-P75616
Handling Instructions
Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized human CD155/Biotin at 10 μg/mL(100 μL/well) can bind human DNAM1-Fc with a linear range of 1.28-20 μg/mL.
Species

Human

Source

HEK293

Tag

C-His

Accession

P15151 (M1-N343)

Gene ID
Synonyms
Poliovirus receptor; Nectin-like protein 5; NECL-5; CD155; PVR; PVS
Molecular Weight

Approximately 36.5 kDa

Purity

Greater than 95 % as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
PVR/CD155 Protein, Human (Biotinylated, HEK293, His)
Cat. No.:
HY-P75616
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